| RESEARCH PAPERS |
|
|
|
|
| Influence of Mouse Embryonic Stem Cells on Melanoma Cells in vitro |
| ZHANG Xiao-mei, FANG Liao-qiong, WANG Zhi-biao |
| State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology Chongqing Key Laboratory of Ultrasound in Medicine and Engineering Department of Biomedical Engineering Chongqing Medical University, Chongqing 400016, China |
|
|
|
Abstract To investigate the impacts of mouse embryonic stem cells on mouse melanoma B16 cells in vitro co-cultured environment. C57BL / 6 mouse embryonic stem cell lines were established.The effects of mouse embryonic stem cells on tumor cell morphology and growth behavior were observed with establishing a co-culture system in vitro.MTT assay and transwell chamber assay were used to detect change of the co-cultured tumor cell adhesion, migration and invasiveness. Mouse embryonic stem cells could invade and push the mouse melanoma cells to form their own room for growth in co-culturing. The adhesion, migration and invasion ability of the tumor cells after co-cultured with mouse embryonic stem cells significantly declined(P<0.05,P<0.01). The results implied that the co-culture mouse embryonic stem cells could invade tumor cells and inhibit their related malignant behaviors like adhesion,migration and invasion ability.
|
|
Received: 26 March 2012
Published: 25 June 2012
|
|
|
|
|
[1] Stoika R S, Panchuk R R, Stoika B R.The similarities and the differences of embryogenesis and carcinogenesis.Ontogenez,2004,35:85-90.
[2] Papaioannou V E. Ontogeny, pathology, oncology.Int J Dev Biol,1993,37: 33-37.
[3] Jurgens B, Schmitz-Drager B J, Schulz W A.Hypomethylation of L1 LINE sequences prevailing in human urothelial carcinoma.Cancer Res,1996,56:5698-5703.
[4] 后晓南,谭毅,赵劼,等.小鼠囊胚与肿瘤细胞共培养时的生长行为观察.第三军医大学学报,2001,23 (8): 954-956. Hou X L,Tan Y,Zhao J,et al.Growth behavior of mouse blastocyst co-cultured with tumor cell line-A549.Acta Academiae Medicinae Militaris,2001,23 (8): 954-956.
[5] 张弘,丁晓燕,方廖琼,等.小鼠囊胚与黑色素瘤细胞共培养模型优化.中国细胞生物学学报,2010,32(5):1-4. Zhang H,Ding X Y,Fang L Q,et al.A optimized co-culture model of mouse blastocysts and melanoma cells.Chinese Journal of Cell Biology,2010,32(5):1-4.
[6] Chen H,Qian K,Zhang S M,et al,Effect of mouse embryonic fibroblasts of Kunming white mice of different inoculating densities on human embryonic stem cells. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu,2007,11(3):443-446.
[7] Carmichael J, Degrall W G, Gazdar A F,et al.Evaluation of a tetrazolium based semiautomated colorimetric assay:assessment of chemosensitivity testing.Cancer Res,1987,47(4):936-942.
[8] Postovit L M,Seftor E A, Seftor R E,et al.A three-dimensional model to study the epigenetic effects induced by the microenvironment of human embryonic stem cells.Stem Cells,2006,24(3):501-505.
[9] Miller S J,Lavker R M,Sun T T.Interpreting epithelial cancer biology in the context of stem cells:Tumor properties and therapeutic implications.Biochim Biophys Acta,2005,1752 (1):25-52.
[10] Postovit L M,Seftor E A, Seftor R E,et al.Influence of the microenvironment on melanoma cell fate determination and phenotype.Cancer Res,2006,66(16):7833-7836.
[11] Lee L M,Seftor E A,Bonde G,et al.The fate of human malignant melanoma cells transplanted into zebrafish embryos:assessment of migration and cell division in the absence of tumor formation.Dev Dyn,2005,233(4):1560-1570.
[12] Kulesa PM,Kasemeier-Knlesa J C,Teddy J M,et al.Reprogramming metastatic cells to assume a neural crest cell-like phenotype in an embryonic microenvironment.Proc Natl Acad Sci USA,2006,103(10): 3752-3757.
[13] Singh H,Nardo L,Kimber S J,et a1.Early stages of implantation as revealed by an in vitro model.Reproduction,2010,139(5):905-914.
[14] Hanahan D,Weinberg R A.The hallmarks of cancer.Cel1,2000,100(1):57-70.
[15] 王焕英,赵勃,壬嫒,等.与小鼠囊胚共培养后人子宫内膜癌细胞侵袭、粘附和运动性的变化.重庆医科大学学报,2005,30(1):9-11. Wang H Y,Zhao B,Ren Y,et a1.Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro.Journal of Chongqing Medical University,2005,30(1):9-11.
[16] Postovit L M,Margaryan N A,Seftor E A, et al.Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells.Proc Natl Acad Sci USA,2008,105(11):4329-4334. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
