The Effects of LncRNA SNHG3 on the Proliferation, Migration and Invasion of Human Breast Cancer MCF-7 Cells

doi:10.13523/j.cb.20190102

China Biotechnology

2019, Vol. 39

Issue (1): 13-20 DOI: 10.13523/j.cb.20190102

The Effects of LncRNA SNHG3 on the Proliferation, Migration and Invasion of Human Breast Cancer MCF-7 Cells

Qun WAN,Meng-yao LIU,Jing XIA,Li-yao GOU,Min TANG,Shi-lei SUN,Yan ZHANG(

)

Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education,Chongqing Medical University, Chongqing 400016, China

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Abstract

Objective:To investigate the effects of LncRNA SNHG3 on the proliferation, migration and invasion of human breast cancer MCF-7 cells.Methods:The recombiant plasmid of SNHG3 was constucted. The experiment was divided into two group: control group (transfected with pcDNA-3.1+plasmid) and treatment group (transfected with pcDNA-3.1+/SNHG3 plasmid). The mRNA level of SNHG3 was detected by qRT-PCR; The mRNA and protein levels of MMP9, EMT-related makers were measured by qRT-PCR and Western blot; The colony formation assay was used to test the proliferation of MCF-7 cells; The wound- healing and Transwell chamber assay were taken to evaluate the migration and invasion of MCF-7 cells.Results:The expression of SNHG3 in MCF-7 cells was highly upregulated compared with control group(P<0.001); Furthermore, The mRNA and protein levels of MMP9 and EMT-related markers were increased; The migration and invasion abilities of SNHG3 overexpression group measured by wound healing assay and transwell chamber assay were augmented significantly. And the colony formation assay showed that proliferation of treatment group were increased remarkably.Conclusion:Overexpression of SNHG3 may promote the proliferation, migration and invasion of breast cancer MCF-7 cells by activating the EMT signaling pathway.

Key words： Breast cancer LncRNA SNHG3 Proliferation Migration Invasion

Received: 03 September 2018 Published: 28 February 2019

ZTFLH:

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Corresponding Authors: Yan ZHANG E-mail: zy2753@hotmail.com

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	Qun WAN
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	Li-yao GOU
	Min TANG
	Shi-lei SUN
	Yan ZHANG

Cite this article:

Qun WAN,Meng-yao LIU,Jing XIA,Li-yao GOU,Min TANG,Shi-lei SUN,Yan ZHANG. The Effects of LncRNA SNHG3 on the Proliferation, Migration and Invasion of Human Breast Cancer MCF-7 Cells. China Biotechnology, 2019, 39(1): 13-20.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190102 OR https://manu60.magtech.com.cn/biotech/Y2019/V39/I1/13

Table 1 Primer sequence

Fig.1 Differential expression of SNHG3 in breast cancer and mammary tissue in Richardson breast 2 database P<0.01 (breast vs breast carcinoma)

Fig.2 SNHG3 overexpressed in breast cancer MCF-7 cells The expression of SNHG3 mRNA in MCF-7 cells was detected by qRT-PCR *** : P<0.001(pcDNA3.1+ vs. pcDNA3.1+/SNHG3)

Fig.3 SNHG3 promoted the proliferation of MCF-7 cells The MCF-7 cells proliferation were determined by colony forming test ** :P<0.01 (pcDNA3.1+ vs. pcDNA3.1+/SNHG3)

Fig.4 SNHG3 promoted the migration of MCF-7 cells (a),(b) The MCF-7 cells migration were determined by wound healing test and Transwell migration assay ** : P<0.01(pcDNA3.1+ vs. pcDNA3.1+/SNHG3); *** : P<0.001(pcDNA3.1+ vs. pcDNA3.1+/SNHG3)

Fig.5 SNHG3 promoted the invasion of MCF-7 cells The MCF-7 cells invasion were determined by Transwell invasion assay *** : P<0.001(pcDNA3.1+ vs. pcDNA3.1+/SNHG3)

Fig.6 SNHG3 activated the EMT signaling pathway of MCF-7 cells (a) The expression of MMP9 and EMT mRNA were detected by qRT-PCR (b) The expression of MMP9 and EMT protein were detected by Western blot * : P< 0.05(pcDNA3.1+ vs. pcDNA3.1+/SNHG3); ** : P< 0.01(pcDNA3.1+ vs. pcDNA3.1+/SNHG3)


[1]	Chan S, Friedrichs K, Noel D , et al. Prospective randomized trial of docetaxel versus doxorubicin in patients with metastatic breast cancer. Journal of Clinical Oncology, 1999,17(8):2341-2354. doi: 10.1200/JCO.1999.17.8.2341

[2]	Lin C, Yang L . Long noncoding RNA in cancer: wiring signaling circuitry. Trends in Cell Biology, 2018,28(4):287-301. doi: 10.1016/j.tcb.2017.11.008

[3]	Terracciano D, Terreri S, De F N , et al. The role of a new class of long noncoding RNAs transcribed from ultraconserved regions in cancer. Biochim Biophys Acta, 2017,1868(2):449. doi: 10.1016/j.bbcan.2017.09.001 pmid: 28916343

[4]	Ballantyne M D, Pinel K, Dakin R , et al. Smooth muscle enriched long noncoding RNA (SMILR) regulates cell proliferation. Circulation, 2016,133(21):2050-2065. doi: 10.1161/CIRCULATIONAHA.115.021019 pmid: 4872641

[5]	Sun T, Wong N . Transforming growth factor-β-induced long noncoding RNA promotes liver cancer metastasis via RNA-RNA crosstalk. Hepatology, 2015,61(2):722-724. doi: 10.1002/hep.27599 pmid: 25380484

[6]	Ranzani V, Arrigoni A, Rossetti G , et al. Next-generation sequencing analysis of long noncoding RNAs in CD4 + T cell differentiation . Methods Mol Biol, 2017,1514:173-185. doi: 10.1007/978-1-4939-6548-9

[7]	Padua D, Mahurkar-Joshi S, Law I K , et al. A long noncoding RNA signature for ulcerative colitis identifies IFNG-AS1 as an enhancer of inflammation. Am J Physiol Gastrointest Liver Physiol, 2016,311(3):G446-G457. doi: 10.1152/ajpgi.00212.2016 pmid: 27492330

[8]	Jia P, Cai H, Liu X , et al. Long non-coding RNA H19 regulates glioma angiogenesis and the biological behavior of glioma-associated endothelial cells by inhibiting microRNA-29a. Cancer Lett, 2016,381(2):359-369. doi: 10.1016/j.canlet.2016.08.009 pmid: 27543358

[9]	Yang F, Zhang H, Mei Y , et al. Reciprocal regulation of HIF-1α and lincRNA-p21 modulates the Warburg effect. Mol Cell, 2014,53(1):88-100. doi: 10.1016/j.molcel.2013.11.004 pmid: 3222224316222

[10]	Zhang T, Cao C, Wu D , et al. SNHG3 correlates with malignant status and poor prognosis in hepatocellular carcinoma. Tumor Biology, 2016,37(2):2379-2385. doi: 10.1007/s13277-015-4052-4 pmid: 26373735

[11]	Chan S, Friedrichs K, Noel D , et al. Prospective randomized trial of docetaxel versus doxorubicin in patients with metastatic breast cancer. Journal of Clinical Oncology, 1999,17(8):2341-2354 doi: 10.1200/JCO.1999.17.8.2341

[12]	Kawaji H, Severin J, Lizio M , et al. Update of the FANTOM web resource: from mammalian transcriptional landscape to its dynamic regulation. Nucleic Acids Research, 2009,10(4):R40. doi: 10.1093/nar/gkq1112 pmid: 21075797

[13]	He H, Wei Z, Du F , et al. Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells. Cellular Signalling, 2017,31:87-95. doi: 10.1016/j.cellsig.2017.01.003 pmid: 28069441

[14]	Tang Y, Xiao G, Chen Y , et al. LncRNA MALAT1 promotes migration and invasion of non-small-cell lung cancer by targeting miR-206 and activating Akt/mTOR signaling. Anti-Cancer Drugs, 2018,29(8):725-735. doi: 10.1097/CAD.0000000000000650

[15]	Wu W, Chen F, Cui X , et al. LncRNA NKILA suppresses TGF-β-induced epithelial-mesenchymal transition by blocking NF-κB signaling in breast cancer. International Journal of Cancer, 2018,143(9):2213-2224. doi: 10.1002/ijc.v143.9

[16]	Cabibbo G, Craxì A . Epidemiology, risk factors and surveillance of hepatocellular carcinoma. European Review for Medical & Pharmacological Sciences, 2010,14(4):352. doi: 10.1016/S0924-977X(10)70001-8 pmid: 20496547

[17]	Chen G G, Ho R L, Wong J , et al. Single nucleotide polymorphism in the promoter region of human alpha-fetoprotein (AFP) gene and its significance in hepatocellular carcinoma (HCC). Eur J Surg Oncol, 2007,33(7):882-886. doi: 10.1016/j.ejso.2007.02.036 pmid: 17433605

[18]	Li N, Zhan X, Zhan X . The lncRNA SNHG3 regulates energy metabolism of ovarian cancer by an analysis of mitochondrial proteomes. Gynecologic Oncology, 2018,150(2):343-354. doi: 10.1016/j.ygyno.2018.06.013 pmid: 29921511

[19]	Hou Z, Xu X, Fu X , et al. HBx-related long non-coding RNA MALAT1 promotes cell metastasis via up-regulating LTBP3 in hepatocellular carcinoma. American Journal of Cancer Research, 2017,7(4):845-856. pmid: 28469957

[20]	Kotiyal S . Bhattacharya Breast cancer stem cells, EMT and therapeutic targets Biochem. Biophys. Res Commun, 2014,453(1):112-116.

[21]	May C D, Sphyris N, Evans K W , et al. Epithelial-mesenchymal transition and cancer stem cells: a dangerously dynamic duo in breast cancer progression. Breast Cancer Research, 2011,13(1):202. doi: 10.1186/bcr2789 pmid: 3109556

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