The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells

doi:10.13523/j.cb.20180502

China Biotechnology

2018, Vol. 38

Issue (5): 10-16 DOI: 10.13523/j.cb.20180502

The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells

Li-yao GOU,Meng-yao LIU,Jing XIA,Qun WAN,Chi-lei SUN,Min TANG,Yan ZHANG(

)

Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education,Chongqing Medical University, Chongqing 400016, China

Download:

HTML

PDF(1393KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

Objective:To investigate the effects of bone morphogenetic protein 9 (BMP9) on the proliferation and migration of human bladder cancer BIU-87 cells.Methods:BIU-87 cells were infected with BMP9 recombinant adenovirus(AdBMP9); The mRNA levels of BMP9 were detected by Q-PCR; The BMP9 protein and its downstream associated signaling pathway protein levels were detected by Western blot; The MTT and colony formation assay were used to test the proliferation of BIU-87 cells; The wound healing and Transwell ^TM chamber assay were taken to evaluate the migration of BIU-87 cells. Results:The expression of BMP9 in BIU-87 cells was highly increased after AdBMP9 infection; Forced expression of BMP9 promotes both the proliferation and migration of BIU-87 cells; Western blot results showed that BMP9 overexpression also increased AKT phosphorylation.Conclusion:High levels of BMP9 may promote the proliferation and migration of bladder cancer BIU-87 cells by activating the AKT signaling pathway.

Key words： Bladder cancer BMP9 BIU-87 cells Cell proliferation Cell migration

Received: 31 August 2017 Published: 05 June 2018

ZTFLH:

Q78

Corresponding Authors: Yan ZHANG E-mail: zy2753@hotmail.com

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Li-yao GOU
	Meng-yao LIU
	Jing XIA
	Qun WAN
	Chi-lei SUN
	Min TANG
	Yan ZHANG

Cite this article:

Li-yao GOU,Meng-yao LIU,Jing XIA,Qun WAN,Chi-lei SUN,Min TANG,Yan ZHANG. The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells. China Biotechnology, 2018, 38(5): 10-16.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180502 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I5/10

Fig.1 Differential expression of BMP9 in normal bladder mucosa and bladder cancer Differential expression of BMP9 in 256 cases of bladder mucosa specimens. 1: Normal bladder mucosa;2:Superficial bladder cancer;3:Infiltrating bladder cancer.; a:P<0.05(group 1 vs.group 2 or 3)

Fig.2 Construction of recombinant BIU-87 cells (a)The expression of BMP9 mRNA in BIU-87 cells was detected by RT-PCR (b) The expression of BMP9 protein was detected by Western blot Blank: Blank control group; AdGFP: Recombinant adenoviruses green fluorescent protein group; AdBMP9: Recombinant adenoviruses BMP9 group; a: P<0.05 (AdBMP9 vs. Blank or AdGFP)

Fig.3 BMP9 promotes the proliferation of BIU-87 cells (a),(b) The BIU-87 cells proliferation were determined by colony forming test and MTT assay a: P<0.05 (AdBMP9 vs. Blank or AdGFP)

Fig.4 BMP9 promotes the migration of BIU-87 cells (a),(b)The BIU-87 cells migration were determined by wound healing test and Transwell migration assay a: P<0.05 (AdBMP9 vs. Blank or AdGFP)

Fig.5 BMP9 activates the AKT signaling pathway of BIU-87 cells (a),(b)The expression and activation of AKT protein were detected by Western blot a: P<0.05 (AdBMP9 vs. Blank or AdGFP)


[1]	Siegel R L, Miller K D, Jemal A . Cancer statistics, 2016. Ca A Cancer Journal for Clinicians, 2016,66(1):7. doi: 10.3322/caac.21332

[2]	韩苏军, 张思维, 陈万青 , 等. 中国膀胱癌发病现状及流行趋势分析. 癌症进展, 2013,11(1):89-95.

[2]	Han S J, Zhang S W, Chen W Q , et al. Analysis of the status and trends of bladder cancer incidence in China. Oncology Progress, 2013,11(1):89-95.

[3]	韩苏军, 张思维, 陈万青 , 等. 中国膀胱癌死亡现状及流行趋势分析. 现代泌尿外科杂志, 2013,18(3):228-232.

[3]	Han S J, Zhang S W, Chen W Q , et al. Analysis of the status quo and trends: mortality in patients with bladder cancer in China, Journal of Modern Urology, 2013,18(3):228-232.

[4]	Alarmo E L, Kallioniemi A . Bone morphogenetic proteins in breast cancer: dual role in tumourigenesis. Endocrine-related Cancer, 2010,17(2):R123.

[5]	Wang K, Feng H, Ren W , et al. BMP9 inhibits the proliferation and invasiveness of breast cancer cells MDA-MB-231. Journal of Cancer Research & Clinical Oncology, 2011,137(11):1687-1696. doi: 10.1007/s00432-011-1047-4 pmid: 21892652

[6]	Herrera B , García-álvaro M, Cruz S, et al. BMP9 is a proliferative and survival factor for human hepatocellular carcinoma cells. PLoS One, 2013,8(7):e69535. doi: 10.1371/journal.pone.0069535 pmid: 3720667

[7]	Kuzaka B, Janiak M, Włodarski K H , et al. Expression of bone morphogenetic protein-2 and -7 in urinary bladder cancer predicts time to tumor recurrence. Archives of Medical Science, 2015,11(2):378-384. doi: 10.5114/aoms.2014.46796 pmid: 25995755

[8]	Soloway M S . Editorial comment on: EAU guidelines on non-muscle-invasive urothelial carcinoma of the bladder. European Urology, 2008,54(2):313. doi: 10.1016/j.eururo.2008.04.052 pmid: 18468783

[9]	van Rhijn B W, Burger M Y . Recurrence and progression of disease in non-muscle-invasive bladder cancer: from epidemiology to treatment strategy. European Urology, 2009,56(3):430. doi: 10.1016/j.eururo.2009.06.028 pmid: 19576682

[10]	Yeung C, Dinh T, Lee J . The health economics of bladder cancer: an updated review of the published literature. Pharmacoeconomics, 2014,32(11):1093-1104. doi: 10.1007/s40273-014-0194-2 pmid: 25056838

[11]	Siegel R, Ma J, Zou Z , et al. Cancer statistics, 2014. Ca A Cancer Journal for Clinicians, 2014,64(1):9. doi: 10.3322/caac.21208

[12]	Xue M, Xu L I, Wenjing W U , et al. Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis. Oncology Reports, 2014,31(5):1993-2000. doi: 10.3892/or.2014.3092 pmid: 4020618

[13]	Jacobs B L, Lee C T, Montie J E . Bladder cancer in 2010: how far have we come. Ca A Cancer Journal for Clinicians, 2010,60(4):244-272. doi: 10.3322/caac.20077 pmid: 20566675

[14]	葛仙伟, 黄后宝 . 膀胱癌相关分子生物学研究现状. 承德医学院学报, 2017,34(4):329-332.

[14]	Ge X W, Huang H B . Current status of molecular biology in bladder cancer. Journal of Chengde Medical College, 2017,34(4):329-332.

[15]	Wu W, Zhang S, Li X , et al. Ets-2 regulates cell apoptosis via the Akt pathway, through the regulation of urothelial cancer associated 1, a long non-coding RNA, in bladder cancer cells. PLoS One, 2013,8(9):e73920-e73920. doi: 10.1371/journal.pone.0073920 pmid: 3771932

[16]	Chen W, Luo K, Ke Z , et al. TBK1 promote bladder cancer cell proliferation and migration via Akt Signaling. Journal of Cancer, 2017,8(10):1892-1899. doi: 10.7150/jca.17638 pmid: 5556653

[17]	Chen Z . B7-H3 promotes the migration and invasion of human bladder cancer cells via the PI3K/Akt/STAT3 Signaling pathway. Journal of Cancer, 2017,8(5):816-824. doi: 10.7150/jca.17759 pmid: 5381170

[18]	马明, 金国江, 付立业 . 上调microRNA-100对结肠癌HT-29细胞增殖和侵袭的影响. 现代肿瘤医学, 2016,24(7):1031-1033. doi: 10.3969/j.issn.1672-4992.2016.07.003

[18]	Ma M, Jin G J, Fu L Y . The effect of up -regulating microRNA -100 in colon cancer HT -29 cells on prolifera-tion and apoptosis. Journal of Modern Oncology, 2016,24(7):1031-1033. doi: 10.3969/j.issn.1672-4992.2016.07.003

[19]	Xu C, Zeng Q, Xu W , et al. miRNA-100 inhibits human bladder urothelial carcinogenesis by directly targeting mTOR. Molecular Cancer Therapeutics, 2013,12(2):207. doi: 10.1158/1535-7163.MCT-12-0273

[20]	Wang J, Zhang X, Wang L , et al. Downregulation of urinary cell-free microRNA-214 as a diagnostic and prognostic biomarker in bladder cancer. Journal of Surgical Oncology, 2015,111(8):992. doi: 10.1002/jso.23937 pmid: 25975233

[21]	Xing J Z, Hua Y, Cheng W Z , et al. Dysregulation of miR-15a and miR-214 in human pancreatic cancer. Journal of Hematology & Oncology, 2010,3(1):46. doi: 10.1186/1756-8722-3-46 pmid: 21106054

[22]	Tao C, Wu C, Hu H . AB266. Expression of long noncoding RNA lncRNA-n336928 is correlated with tumor stage and grade and overall survival in bladder cancer. Biochemical & Biophysical Research Communications, 2015,468(4):666-670. doi: 10.21037/tau.2016.s266 pmid: 4842632

[23]	Zhu H, Li X, Song Y , et al. Long non-coding RNA ANRIL is up-regulated in bladder cancer and regulates bladder cancer cell proliferation and apoptosis through the intrinsic pathway. Biochem Biophys Res Commun, 2015,467(2):223-228. doi: 10.1016/j.bbrc.2015.10.002 pmid: 26449463

[24]	Wang Y, Chen W, Yang C , et al. Long non-coding RNA UCA1a(CUDR) promotes proliferation and tumorigenesis, of bladder cancer. International Journal of Oncology, 2012,41(1):276. doi: 10.3892/ijo.2012.1443 pmid: 22576688

[25]	Liang Z, Wang X, Xu X , et al. MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway. Molecular Cancer, 2017,16(1):96. doi: 10.1186/s12943-017-0664-1 pmid: 28549468

[26]	Li Y, Shan Z, Liu C , et al. MicroRNA-294 promotes cellular proliferation and motility through the PI3K/AKT and JAK/STAT pathways by upregulation of NRAS in bladder cancer. Biochemistry Biokhimiia, 2017,82(4):474. doi: 10.1134/S0006297917040095 pmid: 28371605

[27]	Wu W, Zhang S, Li X , et al. Ets-2 regulates cell apoptosis via the Akt pathway, through the regulation of urothelial cancer associated 1, a long non-coding RNA, in bladder cancer cells. PLoS One, 2013,8(9):e73920-e73920. doi: 10.1371/journal.pone.0073920 pmid: 3771932

[1]	LU Yu-xiang,LI Yuan,FANG Dan-dan,WANG Xue-bo,YANG Wan-peng,CHU Yuan-kui,YANG Hua. The Role and Expression Regulation of MiR-5047 in the Proliferation and Migration of Breast Cancer Cells[J]. China Biotechnology, 2021, 41(4): 9-17.

[2]	XU An-jian,LI Yan-meng,WU Shan-na,ZHANG Bei,YAO Jing-yi. PHP14 Plays a Role in Epithelial-Mesenchymal Transition of AML-12Cell Through Interaction with Vimentin[J]. China Biotechnology, 2021, 41(2/3): 1-6.

[3]	HUANG Sheng, YAN Qi-tao, XIONG Shi-lin, PENG Yi-qi, ZHAO Rui. *Construction of CHD5* Gene Overexpressing Lentiviral Vector Based on CRISPR/Cas9-SAM System and the Effect of CHD5 on Proliferation, Migration and Invasion in T24 Cells**[J]. China Biotechnology, 2020, 40(3): 1-8.

[4]	YANG Dan,TIAN Hai-shan,LI Xiao-kun. Research Progress of Fibroblast Growth Factor 5[J]. China Biotechnology, 2020, 40(3): 117-124.

[5]	Yan-ni HAO,Ting LI,Jia-xin FAN,Luo LI,Ling-fang NIU,Li-ping OU,Xiao-hou WU,Chun-li LUO. Effects of shPLCε on Warburg Effect Through CDC25A in T24 Cells[J]. China Biotechnology, 2018, 38(5): 33-39.

[6]	Yi-man LI,Qin ZHOU. *The Effects of Herpud1* on Metanephric Mesenchymal Cells and Its Mechanism**[J]. China Biotechnology, 2018, 38(3): 9-15.

[7]	FENG Yuan, TANG Yun, XU Lei, TAN Hai-gang. Algal Polysaccharides Inhibits Proliferation and Migration of Liver Cancer Cell Hep3B Via Down-regulation of EMP Pathway[J]. China Biotechnology, 2017, 37(9): 31-40.

[8]	JI Cai-xia, LIU Xiao-hua, XU Li, DONG Chao-qun, LUO Jin-yong. Runx1 Enhances BMP9-induced Osteogenic Differentiation in Mesenchymal Stem Cell Line MEFs[J]. China Biotechnology, 2017, 37(3): 10-17.

[9]	ZHAO Yan, HAO Yan-ni, LIU Nan-jing, LI Ting, WU Xiao-hou, LUO Chun-li. Silence of PLCε Induced by miR-145 Inhibits EMT and Metastasis in Bladder Cancer[J]. China Biotechnology, 2017, 37(3): 27-36.

[10]	LIANG Shan, JIANG Zi-chuan, FENG Jun. The Effect on NCI-N87 Cell Proliferation and Invasion by Carmine Radish Anthocyanin[J]. China Biotechnology, 2017, 37(11): 101-108.

[11]	QIN Hai-xia, CUI Hong-kai, PAN Ying, HU Rui-li, ZHU Li-hong, WANG Shi-jin. miR-335 Regulate Cell Proliferation by Targeting Rho Associated Coiled-coil Forming Protein Kinase 1 in Ovarian Cancer Cells SKOV3[J]. China Biotechnology, 2016, 36(6): 24-31.

[12]	TANG Da-gang, WANG Miao, ZHANG Yan-liang, WANG Xiao-lin, LI Gui-qiang, LUO Xiao-ji. Hey1 Gene Is Involved in Regulating BMP9-induced Osteogenic Differentiation of C3H10T1/2 Cells[J]. China Biotechnology, 2015, 35(6): 14-20.

[13]	WU Rui, ZHOU Lan, CUI Fang. S100A9 Promotes Human Hepatocellular Carcinoma Cell HepG2 Proliferation and Invasion Involving RAGE[J]. China Biotechnology, 2015, 35(5): 8-14.

[14]	LI Li, MENG Qiu-rong, GUO Qi, WANG Lan, SHANG Lei, OU Xin-ying, LUO Jin-yong. Shh Signaling is Involved in Regulating BMP9-induced Osteogenic Differentiation of Mesenchymal Stem Cells[J]. China Biotechnology, 2014, 34(9): 9-15.

[15]	LIU Xue-jie, LIN Wei-ran, TANG Li-chun, SUN Wei, WEI Han-dong, JIANG Ying. *Construction of Lentiviral Expression Vector Expressing Human RAB27A* and Investigation Its Effect on the Proliferation of HepG2 Cell Lines**[J]. China Biotechnology, 2014, 34(9): 16-23.

Viewed

Full text

Abstract

Cited

Shared

Discussed