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| The Effect on NCI-N87 Cell Proliferation and Invasion by Carmine Radish Anthocyanin |
| LIANG Shan, JIANG Zi-chuan, FENG Jun |
| College of Life & Tech, Yangtze Normal University, Chongqing 408100, China |
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Abstract To explore the extraction technology of anthocyanin from carmine radish, five single factors were studied including solid-liquid ratio, digestion temperature, digestion time, ultrasonic power, and ultrasonic power. MTT and TranswellTM were used to discuss the proliferation, invasion ability of human gastric carcinoma cells(NCI-N87)which effect by anthocyanin from carmine radish. Furthermore, Western blot used to analyze the HER2 pathway. The optimum extraction technology was determined based on response surface analysis:1% acid-ethanol as extracting agent, 1:10 solid-liquid ratio, digestion 2h at 40℃ with 400W ultrasonic disruption for 15min. Under this condition, anthocyanin extraction content was 3.92mg/g. 30μg/ml anthocyanin from carmine radish inhibited NCI-N87 cell proliferating and invasion ability, decreased HER and p-Akt expression.
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Received: 05 July 2017
Published: 15 November 2017
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