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| The Types of siRNA Off-target Effects and the Strategies for Mitigation |
| TANG De-ping1, MAO Ai-hong2, LIAO Shi-qi2, XUE Lin-gui1, ZHANG Bing-lin1 |
1. The School of Chemical & Biological Engineering, Lanzhou Jiaotong University, lanzhou 730070, China;
2. Institute of Gansu Medical Science Research, lanzhou 730050, China |
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Abstract Small interfering RNAs (siRNAs) can specific silence target genes,and are widely used to elucidate gene function,indentify drug targets and develop more specific therapeutics than are currently available. Off-target effects (OTEs) can complicate the interpretation of phenotypic effects in gene-silencing experiments and can potentially lead to unwanted toxicities. siRNAs OTEs include microRNA-like off-target effects, immune stimulation and saturation of the RNAi machinery. The types of off-target effects of siRNAs and methods to mitigate them was focused, to help enable effective application of RNAi technology.
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Received: 21 February 2012
Published: 25 July 2012
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