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siRNA Inhibits the Growth and Migration of Mouse Melanoma by MMP-9 and FAK Gene |
HU Na1, LIU Qing1, TANG Zhao-yong1, TANG He-jing1, AO Lan1, ZHAO Zi-hao1, FANG Liao-qiong1,2 |
1. College of Biotechnology, Southwest University, Chongqing 400716, China;
2. College of Biomedical Engineering, Chongqing Medical University State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science, Chongqing 400016, China |
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Abstract Objective:To observe the effect of combined-silencing MMP-9 and FAK on migration of malignant melanoma highly metastatic B16F10 cells in vivo. Methods:Construct recombinant plasmid vectors pGV102-MMP9-siRNA and pGV102-FAK-siRNA, then Lipofectamine TM2000 mediated the plasmid vectors were transfected into B16F10 cells. To observe the growth of the tumor, the C57BL/6 mouse model of subcutaneous transplantation was established, and the pathological characteristics of tumor tissue was observed by H&E staining. B16F10 cells were stably intravenously injected into C57BL/6 mice by 5×105. After 24 days of injection, the number of mouse lung metastasis nodules was counted to evaluate the migration ability of tumor cells in vivo. Results:RT-PCR results showed that the mRNA level of MMP-9 and FAK in the transfected cells was significantly reduced than that of normal cells(P<0.01). Compared with the normal cells, the tumor growth rate and the number of lung metastasis nodules of C57BL/6 mice in the transfected cells were significantly reduced(P<0.01). Conclusion:Interference MMP-9 and FAK can significantly inhibit malignant tumor growth and metastasis in vivo.
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Received: 30 November 2015
Published: 25 May 2016
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