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| Effect of Silencing c2orf68 Gene on the Proliferation of Colorectal Adenocarcinoma Cells |
| JIANG Ting-ting, WEN Xiao-xia, CHEN Yao |
| Department of Anatomy, Basic Forensic Medicine College, West China Medical Center of Sichuan University, Chengdu 610041, China |
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Abstract Objective:To explore the c2orf68 gene expression of human colorectal adenocarcinoma SW480,SW620 and LS174T cell lines. Further more, to observe the change of their biological characteristics in three cell lines by applying RNA interference technique to silence c2orf68 and to clarify its in the pathogenesis of colorectal cancer. Methods:Using siRNA online design software designed and synthesised of siRNA interference fragments, and transported them to the three cell lines. The mRNA expression of the c2orf68 were detected by RT-PCR, the cell proliferation was checked by MTT, and the cell apoptosis was measured by flow-cytometry. Results: The mRNA expression of the c2orf68 was inhibited by the two pairs siRNA in SW480,SW620 and LS174T cell lines. It showed that the inhibition rates were as follows: 50.05%,50.05%; 49.69%,49.69%; 50.15%,45.79%. Then, siRNA1 of high interference efficiency was selected. The cell proliferation inhibitory rate was 21.2% in LS174T cell line(P<0.05).The cell apoptosis rate was 18.13% in LS174T cell line(P<0.05).Conclusions: The successfully obtained siRNA fragement which specifically degraded its c2orf68 mRNA, inhibited the growth of the test cells, promoted cell apoptosis. It indicated that c2orf68 might be associated with the proliferation on colorectal cancer cells, its pathogenesis might be through gene expression, further regulated the change of the corresponding biological characteristics, and caused the occurrence of colorectal cancer.
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Received: 18 November 2013
Published: 25 February 2014
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[1] Parkin D M, Pisani P, Ferlay J. Cancer, estimates of the worldwide incidence of eighteen major cancers in 1985.Int J Cancer, 1993,54(4):594-606.
[2] Parkin D M, Bray F, Ferlay J, et al. Global cancer statistics. CA Cancer J Clin, 2005, 55(2):74-108.
[3] Chen Y, Zhang Y Z,Zhou Z G,et al. Identification of differently expressed genes in human colorectal adenocarcinoma. World J Gastroenterol,2006,12(7):1025-1032.
[4] Chen Y, Zhang Y Z. The construction of cDNA suppression subtractive library of human rectum adenocainomas.US Chin J Lymphol Oncol,2002,1(1):9-14.
[5] Wang K C, Chen Y. Analysis of the novel protein in human colorectal adenocarcinoma. Mol Med Report, 2013,8: 529-534.
[6] Jiang Q, Zhang C, Chen Y. NM_001013649.3 gene is down-regulated in human colorectal adenocarcinoma. Mol Med Report, 2011,4(6), 1279-1281.
[7] Fire A,Xu A Q,Montgomery M K,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature,1998,391:806-811.
[8] Fire A.RNA-triggered gene silencing.TIG,1999,15(9):358-363.
[9] Holen T, Amarzguioui M, Wiiger M T, et al. Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor.Nucheic Acids Res,2002,30(8):1757 -1766.
[10] Majid S, Saini S, Dahiya R. Wnt signaling pathways in urologogical cancers: past dacades and still growing. Mol Cancer, 2012, 11: 7.
[11] Xue H H, Zhao D M. Regulation of mature T cell responses by Wnt signaling pathway. Ann N Y Acad Sci, 2012, 1247(1): 16-33.
[12] Cadigan K M. TCFs and Wnt/β-catenin signaling more than one way to throw the switch. Curr Top Dev Biol, 2012, 98: 1-34.
[13] Xiaoxia Wen,Lei Dong,Jianjun Zhu, et al. hcrn81 is upregulated by rapamycin treatment in human colorectal adenocarcinoma. Mol Med Report, 2013,7: 1257-1260.
[14] Fire A,Xu S,Montgomery M K,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.Nature,1998,391:806-811.
[15] Kurrek J. Antisese teehnologis. improvement through novel chemieal Modifications.Eur J Biochem.2003,270(8):1628-1644.
[16] Boutla A, Delidakis C, Livadaras I,et al. Variations of the 3' protruding ends insynthetic short interfering RNA(siRNA) tested by microinjection in Drosphila embryos. Olignucleotides, 2003,13(5):295-301.
[17] Carmell M A,Zhang L, Conklin D S,et al. Rational siRNA design for RNA interference.Nat Biotechnol,2004,22(3):326-330. |
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