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| The Effect of KAT Gene Silencing by RNAi on the Synthesis of 10-HDA in Bees |
| LIU Li, YANG Xiao-hui, WANG Rui-ming |
| Department of Biology Engineering, QILU University of Technology, Jinan 250353, China |
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Abstract The double-stranded RNA of KAT gene was synthesized for RNA interference (RNAi)in bees. After RNAi, the expression of KAT gene and the synthesis of 10-HDA were detected respectively by semi-quantitative RT-PCR and HPLC in order to investigate the relationship between the silencing of KAT gene and the amount of 10-HDA. These results showed that the bees which were injected with 2μl dsKAT had the highest survival rate. And when the bees were injected for five days,the KAT mRNA levels seemed the lowest and the secretion of 10-HDA in honeybee workers showed a significant decrease(P<0.05)relative to the control group which was injected with DEPC water.And after knockdown of KAT, the glands of the bees MGs appeared to be wizened and loose .The KAT gene in bees was silenced successfully,which speculated that KAT might be involved in the biosynthetic pathway of 10-HDA and described the relationship between KAT gene and the synthesis of 10-HDA. It provides a reference for analysing the function of other genes in honeybees by RNAi, and has laid a foundation for the further perfection of 10-HDA biosynthetic pathway. In addition, it provides the basis for improving the yield of 10-HDA by increasing the expression of genes.
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Received: 31 December 2015
Published: 15 February 2016
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