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| Cloning and Expression Analysis of CkLEA1 Gene in Caragana korshinskii Kom |
| YANG Qi, YIN Jia-jia, WANG Yin, WANG Rui-gang, LI Guo-jing |
| College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China |
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Abstract Under stress conditions, a lot of genes responsive to stress will be induced in plants. Late-embryogenesis-abundant proteins (LEA protein) belong to an important protein family related to drought, cold and other abiotic stress. An LEA encoding gene was isolated from the suppression subtractive hybridization (SSH) library of Caragana korshinskii Kom. under drought stress. Sequence and phylogenetic analysis indicated that it belongs to the LEA3 gene family, and was named CkLEA1 (GenBank accession no. KC309408). The full length gDNA of CkLEA1 was 496bp, contained two exons and one intron. The full length cDNA was 357bp, and the protein deduced from this cDNA comprised 99 amino acids. Real-time quantitative PCR analysis showed the transcript of CkLEA1 was induced under different stresses, including drought, cold, heat, salt, ABA and high pH. These results indicate that CkLEA1 might be involved in the stress response of Caragana korshinskii Kom.
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Received: 31 January 2013
Published: 25 May 2013
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