LI Fei-fei, FANG Jing, MA Qiong, FU Hui, MAO Jian-ping
China Biotechnology. 2013, 33(5): 22-27.
Objective: To investigate the natural broneol liquid induce cancer cells apoptosis. Methods:Cells of human nasopharyngeal carcinoma, lung squamous cell carcinoma, lung adenocarcinoma and breast cancer lines were employed. Cells cultured in normal conditions but with medium diluted natural broneol liquid. At different dilutions, different time, cultured cells were detected the proliferation by MTT assay. Death cells were histologically observed on slides. Apoptosis cells were analysed on flow cytometry. Results: Cells cultured in half diluted natural broneol liquid were all quickly lead to apoptosis, and showed apoptosis apparently from 12hrs in quarter diluted natural broneol liquid. The obviousness of apoptosis showed order from breast cancer, lung squamous cell carcinoma, lung epithelial cancer and rhinitis cancer, to normal cells 293T. While 293T only had basal levels of apoptosis. In the culture, withdrawal of the borneol fluid led normal cells quickly restored to growth state, while the cancer cells continue to undergo apoptosis within a certain time. Histologically observed the apoptosis cells showed often the bubbling or sprouting of membrane, nuclear condensed and splitted, cytoplasma reduced, and cell fragmentation. The phosphatidylserine eversion obviously lead to apoptosis early, and Caspases 3 showed changes happened, which induced the cancer cell apoptosis of borneol. Conclusion: It showed that borneol induced cancer cells apoptosis.
