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| Sequencing and Analysis of the Transcriptome of Ginkgo biloba L. Cells |
| ZHANG Nan1, SUN Gui-ling2, DAI Jun-gui3, YANG Yan-fang1, LIU Hong-wei1, QIU De-you1 |
1. State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;
2. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
3. State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China |
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Abstract To sequence the transcriptome of Ginkgo biloba L. cells, Illumina Genome Analyzer IIx was used. One purpose is to discover candidate genes involved in ginkgolide biosynthesis and new hydroxylases in Ginkgo biloba cells such as taxoid 9-alpha hydroxylase, which will complete the unknown hydroxylation steps in taxol biosynthesis pathway in Taxus sp. A total of 69 286 contigs, 56 387 scaffolds and 32 032 unigenes with average length of 636bp were generated. Unigene qualities from several aspects like gap distribution, GC content, gene coverage were assessed. The results indicate that the sequencing data is good with high quality and reliability. Analyzed the information of unigene expression and functional annotation, we found that 66 unigenes belong to CYP450 gene family, 726 relate to secondary metabolism among which 59 involved in terpenoid metabolism and 17 involved in diterpenoid biosynthesis. At last, 15 hydroxylase candidates were selected by bioinformatics analysis our transcriptome data of Ginkgo biloba L. cells and the Michigan State University transcriptome data of Ginkgo biloba L. tissues. These candidate genes selection work set foundation for the further research.
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Received: 15 January 2013
Published: 25 May 2013
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