Study of Co-encapsulated Doxorubicin and Curcumin Poly(butyl cyanoacrylate) Nanoparticles and Reversion of Multidrug Resistance in MCF/ADR Cell Line

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China Biotechnology ›› 2013, Vol. 33 ›› Issue (5) : 35-43.

WU Jun¹, YAN Xin¹, SHAO Rong¹, DUAN Jing-hua²

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Abstract

Co-encapsulated doxorubicin and curcumin in PBCA nanoparticles were prepared with emulsion polymerization. The mean particle size and the zeta potential of DOX-CUR-PBCA-NPs were 133 ? 5.34nm in diameter and +32.23 ? 4.56 mV, respectively. The entrapment efficiencies of DOX and CUR were 49.98 ? 3.32% and 94.52 ? 3.14%, respectively. Anticancer activities and reversal efficacy of the formulations and various combination approaches were assessed using MTT assay and western blotting. The results showed that the dual-agent loaded PBCA-NPs system had the similar cytotoxicity to co-administration of two single-agent loaded PBCA-NPs (DOX-PBCA-NPs + CUR-PBCA-NPs), which was slightly higher than that of the free drug combination (DOX-CUR) and one free drug/another agent loaded PBCA-NPs combination (DOX+CUR-PBCA-NPs or CUR+DOX-PBCA-NPs). The simultaneous administration of DOX and CUR could achieve the highest reversal efficacy, and down-regulate of P-gp in MCF-7/ADR cell lines. Multidrug-resistant can be enhanced by treated combination of encapsulated cytotoxic drugs and reversal agents. Co-encapsulation of anticancer drug DOX and reversal agent CUR can be more effective in reversing multi-drug resistance than the other formulations and might cause lower normal tissue drug toxicity and fewer drug-drug interactions.

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Poly(butyl cyanoacrylate) nanoparticles / Curcumin / Doxorubicin / Adriamycin-resistant human breast carcinoma cell line / Multidrug resistance

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WU Jun, YAN Xin, SHAO Rong, DUAN Jing-hua. Study of Co-encapsulated Doxorubicin and Curcumin Poly(butyl cyanoacrylate) Nanoparticles and Reversion of Multidrug Resistance in MCF/ADR Cell Line[J]. China Biotechnology, 2013, 33(5): 35-43

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