The Protective Effects of DJ-1 Against Rotenone Induced Dopaminergic Neuron Injury in the Rat SN

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China Biotechnology ›› 2013, Vol. 33 ›› Issue (5) : 7-12.

SUI Lei-ming¹, GAO Hua², GU Li³, YANG Wei-wei³, YANG Hui³

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Abstract

Objective: To observe protective effects of DJ-1 against rotenone induced dopaminergic neuron injury in rat substantia nigra (SN). Methods: DJ-1 gene was reconstructed into adeno-associated virus (AAV) vector. Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase (TH) protein was determined using immunohistochemical staining and Western blot. Ethovison software allowed measurement of movement distance in 30 minutes. Rotenone-induced depression-like behaviors in rats was measured by swimming. Results: The animals of the AAV-DJ-1 group significantly increased movement distance and swimming time comparing to control one. Furthermore, overexpression of DJ-1 dramatically inhibited the loss of TH-positive neurons. Conclusion: Overexpression of DJ-1 protected the dopaminergic neurons against rotenone induced injury in the rat SN, and strongly support the potential of DJ-1 gene on drug therapy for PD.

Key words

DJ-1 / Rotenone / Tyrosine hydroxylase / Parkinson’s disease

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SUI Lei-ming, GAO Hua, GU Li, YANG Wei-wei, YANG Hui. The Protective Effects of DJ-1 Against Rotenone Induced Dopaminergic Neuron Injury in the Rat SN[J]. China Biotechnology, 2013, 33(5): 7-12

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