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Construction of Recombinant Lacbacillus rhamnosus with Tetracycline Resistance |
GUO Ling, SUN Liang, LI Jian-xiu, SUN Fei-fei, HUANG Yan-yan, HUANG Ri-bo |
State Key Laboratory of Non-Food Biomass and Enzyme Technology,National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Science,Guangxi Key Laboratory of Biorefinery,Nanning 530007,China |
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Abstract The suicide plasmid pUC-ldhD-Ter containing homologous sequence from Lacbacillus rhamnosus was transformed into Lactobacillus rhamnosus JCM 1553,Two recombinant stains have been constructed successfully and named GL-1,GL-2. PCR was applied to clone the gene of tetracycline from the chromosomal DNA of recombinant stains. The growth curve of the recombinant Lacbacillus rhamnosus was consistent with that of the wild-type strain.The highest yield of L-lactic acid in Erlenmeyer flasks from 10% glucose at 37℃/200 r/min by GL-1 and GL-2 are 93.956 g/L,and 93.693 g/L after 40h.The conversion of glucose are 94.82% and 94.56%. OD600 value of the two strains achieve 25.49 and 24.66.The residual sugar content are 0.60% and 0.63% respectively.The result showed no remarkable difference from the wild type.
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Received: 15 March 2011
Published: 25 August 2011
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