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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (03): 67-73    DOI:
    
Optimization of Fermentation Condition for a Cold-active Lipase and Enzymatic Characterization
1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
2.Jiangsu Hanbon Science & Technology Co. Ltd, Huai’an 223001, China
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Abstract  

The medium and condition of liquid fermentation for the yield of lipase of Burkholderia sp. SYBC LIP-Y were optimized with single factor experiment and orthogonal design. The most suitable medium was formulated with the ingredients: 10 g/L tragantine, 15 g/L beef extract, 0.252 g/L NaNO3, 40 ml/L olive oil, 10 ml/L Triton x-100, pH 7.5, and 10% (V/V) of the inoculum size. The lipase activity reached 83.85 U/ml, which was 3.63 folds of that before optimization. Properties of the lipase after purification by aqueous two-phase system was studied. The optimum pH and temperature of the purified enzyme were 10.0 and 30℃ respectively. About 80% of the original activity is maintained by heating at 40℃ for 60 minutes. The lipase belonged to the cold-active lipase. Good temperature stability and resistant to alcohol were proved. There had wide application fields that can be believed.

Key words： Cold-active lipase      Optimization      Enzymatic property     
Received: 15 September 2009      Published: 25 March 2010
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Articles by authors
YU Bo-Hua
CA Yu-Jie
LIAO Xiang-Ru
YUN Li-Gong
TUN Hui-An
ZHANG Da-Bing
Cite this article:

YU Bo-Hua, CA Yu-Jie, LIAO Xiang-Ru, YUN Li-Gong, TUN Hui-An, ZHANG Da-Bing. Optimization of Fermentation Condition for a Cold-active Lipase and Enzymatic Characterization. China Biotechnology, 2010, 30(03): 67-73.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I03/67

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