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中国生物工程杂志

China Biotechnology
China Biotechnology  2018, Vol. 38 Issue (11): 84-91    DOI: 10.13523/j.cb.20181111
    
Research Progress of Loop-Mediated Isothermal Amplification in Food Safety Testing
Jing WANG,Xin XU,Xue-yu WANG,Lun-guang YAO,Yun-chao KAN(),Jun JI()
Center for Nanyang Veterinary Biological Engineering Technology/Henan Provincial Engineering Laboratory of Insect Bio-reactor,Nanyang Normal University,Nanyang 473061,China
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Abstract  

Food safety has been the focus of public health throughout the world. Loop-mediated isothermal amplification ( LAMP ) technique is a kind of simple and rapid isothermal nucleic acid amplification technology with high specificity, and outstanding sensitivity. Recently, LAMP has been widely evaluated and applied in the field of nucleic-acid detection. LAMP technology can be applied to the food safety detection field, quickly and accurately monitored in a number of food safety problems harm to human health. Therefore, the advantages of LAMP technology in food safety detection are analyzed and combined with the combination of LAMP technology and new diagnostic technology platform.



Key wordsLoop-mediated isothermal amplification      Food safety      Nucleic acid test      Optimization-joint application     
Received: 29 May 2018      Published: 06 December 2018
ZTFLH:  Q52  
Corresponding Authors: Yun-chao KAN,Jun JI     E-mail: lunguangyao@163.com;jijun84@qq.com
Cite this article:

Jing WANG,Xin XU,Xue-yu WANG,Lun-guang YAO,Yun-chao KAN,Jun JI. Research Progress of Loop-Mediated Isothermal Amplification in Food Safety Testing. China Biotechnology, 2018, 38(11): 84-91.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181111     OR     https://manu60.magtech.com.cn/biotech/Y2018/V38/I11/84

Fig.1 Schematic diagram of LAMP primers design
Fig.2 Microdevices of ring mediated isothermal amplification and colloidal gold immunochromatography paper
Fig.3 Digital microfluidics with loop-mediated isothermal amplification
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