电子嗅在线反馈控制毕赤酵母糖化酶发酵过程中甲醇浓度新方法的应用

doi:DOI:10.13523/j.cb.20160310

中国生物工程杂志

2016, Vol. 36

Issue (3): 68-76 DOI: DOI:10.13523/j.cb.20160310

技术与方法

电子嗅在线反馈控制毕赤酵母糖化酶发酵过程中甲醇浓度新方法的应用

施慧琳¹, 孙靖淳¹, 张荣凯¹, 高大启¹, 王泽建¹, 郭美锦¹, 周礼勤², 庄英萍¹

1. 华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心上海生物制造产业技术研究院上海 200237;
2. 广西科学院生物研究所南宁 530007

Application of the Electronic Nose on the Online Feedback Control of Methanol Concentration during Glucoamylase Fermentation Optimization by Pichia pastoris

SHI Hui-lin¹, SUN Jing-chun¹, ZHANG Rong-kai¹, GAO Da-qi¹, WANG Ze-jian¹, GUO Mei-jin¹, ZHOU Li-qin², ZHUANG Ying-ping¹

1. State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology, East China University of Science and Technology, Shanghai 200237, China;
2. Biology Institute, Guang Xi Academy of Sciences, Nanning 530007, China

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摘要：

探索了电子嗅传感仪直接通过发酵尾气进行发酵液中甲醇浓度在线检测的方法,建立了毕赤酵母表达糖化酶过程中甲醇浓度的自动化反馈补料控制模型,可准确实现发酵过程中甲醇浓度的精确控制;研究表明,当利用电子嗅将培养液中甲醇浓度稳定控制在(890±35)ppm水平下,发酵诱导培养到128h时目的蛋白糖化酶酶活达到了8 153U/ml,与甲醇浓度控制在(350±26)ppm时的发酵水平相比提升了48.8%。该方法具有无需前处理、与发酵液非接触、快速和准确性的优点,为提升工程酵母在工业发酵培养过程工艺的优化控制具有重要的指导作用。

关键词： 在线监测; 电子嗅; 甲醇; 毕赤酵母; 糖化酶

Abstract:

A novel method for direct determining the broth methanol concentration online from the fermentation exhausted gas with electronic nose sensor, established an auto-feedback model for methanol concentration control during glucoamylase fermentation by genetic Pichia pastoris were investigated, the model could be used for precise control in the fermentation process. Methanol concentration controlling strategies through electronic nose showed that when the broth methanol concentration in stability controlled at (890±35)ppm, the highest glucoamylase activity reached 8 153U/ml at 128h of fermentation, which was 48.8% higher than that with the methanol concentration controlled at (350±26)ppm. Online methanol detection and feedback control models needn't pre-treatment and contact with the broth, has the rapid and accuracy advantages. It will be approved as an effective and important role on the online methanol concentration analysis and feedback control on industrial enzyme or aim protein fermentation with genetic Pichia pastoris.

Key words: Electronic nose Online determination Methanol Glucoamylase Pichia pastoris

收稿日期: 2015-10-26 出版日期: 2016-01-26

ZTFLH:

Q814.3

基金资助:

国家"863"计划资助项目(2015AA021005)

通讯作者: 庄英萍 E-mail: ypzhaung@ecust.edu.cn

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引用本文:

施慧琳, 孙靖淳, 张荣凯, 高大启, 王泽建, 郭美锦, 周礼勤, 庄英萍. 电子嗅在线反馈控制毕赤酵母糖化酶发酵过程中甲醇浓度新方法的应用[J]. 中国生物工程杂志, 2016, 36(3): 68-76.

SHI Hui-lin, SUN Jing-chun, ZHANG Rong-kai, GAO Da-qi, WANG Ze-jian, GUO Mei-jin, ZHOU Li-qin, ZHUANG Ying-ping. Application of the Electronic Nose on the Online Feedback Control of Methanol Concentration during Glucoamylase Fermentation Optimization by Pichia pastoris. China Biotechnology, 2016, 36(3): 68-76.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/DOI:10.13523/j.cb.20160310 或 https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I3/68

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