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| Human Adipose-derived Mesenchymal Stem Cells Engrafted into Deafened Mammalian Cochlea and Differentiated into Hair Cell-like Cells |
| YUAN Xian-dao1,YAN Xi2,YANG Hua1,GAO Zhi-qiang1,LI Kang-hua2,HAN Qin2,LU Shan2,CHEN Xiao-wei1,QI Fang1,JIANG Hong1,ZHAO Chun-hua2 |
1.Department of Otolaryngology, Peking Union Medical College Hospital， Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
2.Institute of Basic Medical Sciences and School of Basic Medicine，Center of Excellence in Tissue Engineering，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100005，China |
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Abstract Objective: The aim was to examine the potential of human adipose-derived mesenchymal stem cells (hAD-MSCs) transplantation to restore inner ear hair cells in mice. Methods: To test this approach, hAD-MSCs were delivered into the cochlea of deafened mice. The grafted cells were tested by immunostaining and RT-PCR. Results: Transplanted cells survived for at least 2 weeks postoperatively, evidenced by RT-PCR and immunofluorescence detection of hAD-MSCs. A few hAD-MSCs located in the cochlear sensory epithelium, with expression of myosin 7a, a specific marker for inner ear hair cells. There was no evidence of significant immunological rejection of the implanted hAD-MSCs. Conclusion: hAD-MSCs transplanted into the mouse inner ear after drug-induced injury can survive, migrate and differentiate towards hair cell-like cells.
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Received: 21 December 2009
Published: 25 August 2010
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Corresponding Authors:
Robert CHUN-HUA ZHAO
E-mail: zq.gao@yahoo.com;chunhuaz@public.tpt.tj.cn
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Cite this article:
YUAN Xian-Dao, YAN Xi, YANG Hua, GAO Zhi-Jiang, LI Kang-Hua, HAN Qin, LEI Pan, CHEN Xiao-Wei, QI Fang, JIANG Hong, DIAO Chun-Hua. Human Adipose-derived Mesenchymal Stem Cells Engrafted into Deafened Mammalian Cochlea and Differentiated into Hair Cell-like Cells. China Biotechnology, 2010, 30(08): 1-5.
URL:
https://manu60.magtech.com.cn/biotech/Q819 OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I08/1
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