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| Autophagy Protects Against Apoptosis of Human Placental Mesenchymal Stem Cells of Fetal Origin Induced by Tumor Necrosis Fator-α |
ZHU Yongzhao1,**,TAO Jin1,**,REN Meng-meng2,XIONG Ran3,HE Ya-qin1,ZHOU Yu1,LU Zhen-hui1,DU Yong1,YANG Zhi-hong1,**( ) |
1 Ningxia Medical University, Yinchuan 750004, China
2 Yinchuan Maternal and Children Health Care Hospital, Yinchuan 750001, China
3 Shenzhen Gentarget Biopharmaceutical Co., Ltd, Shenzhen 518000, China |
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Abstract Objective: To explore the effects of TNF-α on apoptosis and autophagy of human placenta fetal derived mesenchymal stem cells (hfPMSCs) and the regulation of autophagy on apoptosis.Methods: The expression of CD73, CD90, CD105, CD14, CD34 and CD45 was identified by flow cytometry analysis for fPMSCs cultured in serum-free medium. hfPMSCs were treated with TNF-α at a final concentration of 20 g /L for 24h. Annexin V/PI double staining assay detected cells apoptosis; Each group of total protein was extracted, the expression of autophagy marker protein LC3 Ⅰ/Ⅱ was tested by Western blot; mRFP-GFP-LC3 adenovirus was used to infect fPMSCs, the formation of the puncta light was observed by confocal fluorescence microscopy; hfPMSCs were infected with Atg5 interfering lentivirus (si-Atg5) and negative control lentivirus (si-NC), and Annexin V/PI double staining was used to detect cells apoptosis.Results: The cultured cells possessed typical MSCs morphology and belong to CD73 +CD90 +CD105 +/ CD14 -CD34 -CD45 - cells. Annexin V/PI staining results showed that after TNF-αtreatment for 24 h, the apoptosis number and rate of hfPMSCs were higher than those in the control group (P<0.05). Compared to untreated group, TNF-α increased the deposition number of LC3 Ⅱ (P<0.05),and induced more aggregation of puncta light in cytoplasm.Compared: with the control group, TNF-αinduced apoptosis of hfPMSCs was significantly elevated in autopahgy inhibition group (P<0.05). Conclusion: TNF-αinduced autophagy can inhibit the apoptosis of hfPMSCs, play an important protective function.
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Received: 23 August 2019
Published: 20 September 2019
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Corresponding Authors:
Yongzhao ZHU,Jin TAO,Zhi-hong YANG
E-mail: zhangpac@163.com
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Cite this article:
ZHU Yongzhao,TAO Jin,REN Meng-meng,XIONG Ran,HE Ya-qin,ZHOU Yu,LU Zhen-hui,DU Yong,YANG Zhi-hong. Autophagy Protects Against Apoptosis of Human Placental Mesenchymal Stem Cells of Fetal Origin Induced by Tumor Necrosis Fator-α. China Biotechnology, 2019, 39(9): 62-67.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190909 OR https://manu60.magtech.com.cn/biotech/Y2019/V39/I9/62
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