The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells

doi:10.13523/j.cb.1909030

China Biotechnology

2020, Vol. 40

Issue (4): 17-24 DOI: 10.13523/j.cb.1909030

The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells

TANG Min,WAN Qun,SUN Shi-lei,HU Jing,SUN Zi-jiu,FANG Yu-ting,ZHANG Yan(

)

Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, China

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Abstract

Objective: To investigate the effect of miR-5195-3p on the proliferation, migration and invasion of human cervical cancer cell line SiHa. Methods: The expression levels of miR-5195-3p in human cervical cancer cells SiHa and normal epithelial cells HaCaT were detected by real-time quantitative PCR.The miR-5195-3p mimic was transfected into SiHa cells to construct exogenous overexpressed cell lines,while the negative control group was transfected with NC mimic and the transfection efficiency was verified by real-time quantitative PCR;MTT and colony formation experiments were utilized to assess the proliferation capacity; The competence of transversal migration was examined by Wound healing experiment;Transwell assay was performed to evaluated the longitudinal migration and invasion ability;The mRNA transcription and protein expression levels of E-cadherin, Vimentin and snail were analyzed by real-time quantitative PCR and Western blot. Results: the expression level of miR-5195-3p in SiHa was lower than HaCaT (P<0.05).Compared with the negative control group, the level of miR-5195-3p in SiHa cell which transfected the miR-5195-3p mimic was significantly increased (P<0.01);Moreover, their proliferation,migration and invasion in vitro were distinctly reduced(P<0.001);Meanwhile, the expression levels of E-cadherin were up-regulated while those of Vimentin and snail were down-regulated. Conclusion: overexpression of miR-5195-3p may inhibit the proliferation, migration and invasion of cervical cancer cell SiHa by blocking EMT pathway.

Key words： Cervical Cancer miR-5195-3p Proliferation Migration Invasion

Received: 16 September 2019 Published: 18 May 2020

ZTFLH:

Q291

Corresponding Authors: Yan ZHANG E-mail: zy2753@hotmail.com

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	Min TANG
	Qun WAN
	Shi-lei SUN
	Jing HU
	Zi-jiu SUN
	Yu-ting FANG
	Yan ZHANG

Cite this article:

TANG Min,WAN Qun,SUN Shi-lei,HU Jing,SUN Zi-jiu,FANG Yu-ting,ZHANG Yan. The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells. China Biotechnology, 2020, 40(4): 17-24.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.1909030 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I4/17

Table 1 Primer sequence

Fig.1 The expression of miR-5195-3p in normal epithelial cell HaCaT and cervical cancer cell SiHa The expression of miR-5195-3p in HaCaT cells and SiHa cells was detected by qRT-PCR, * P<0.05(HaCaT vs SiHa)

Fig.2 miR-5195-3p promoted the proliferation of SiHa cells (a)The expression of miR-5195-3p in SiHa cells was detected by qRT-PCR (b)The SiHa cells proliferation were determined by colony forming test (c)The SiHa cells viability were determined by MTT test ** P<0.01, *** P<0.001 (NC vs 5195-3p)

Fig.3 miR-5195-3p promoted the migration of SiHa cells (a),(b) The SiHa cells migration were determined by wound healing test and transwell migration assay * P<0.05, ** P<0.01, *** P<0.001 (NC vs 5195-3p)

Fig.4 miR-5195-3p promoted the invasion of SiHa cells The SiHa cells migration were determined by transwell invasion assay, *** P<0.001 (NC vs 5195-3p)

Fig.5 miR-5195-3p activated the EMT signaling pathway of SiHa cells (a)The expression of EMT mRNA were detected by qRT-PCR (b) The expression of EMT protein were detected by Western blot * P<0.05 (NC vs 5105-3p) ; ** P<0.01 (NC vs 5105-3p)


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