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| Effect of Mechanical Stretch on Migration of Bone Mesenchymal Stem Cells in vitro |
| LI Xiao-na, SONG Guan-bin, LUO Qing |
| College of Bioengineering, Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqing University, Chongqing 400044, China |
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Abstract Objective: To investigate the effects of the cyclic stretch on rat bone marrow mesenchymal stem cells (BMSCs) migration and its molecular mechanism. Methods: The cyclic mechanical stretching apparatus were used to study the effects of the cyclic stretch on the migration of BMSCs with different parameters. Transwell and scratching method was used to detect the number of cell migration and the levels of matrix metallopoteinases-2, -9 proteins were detected by zymography. Results: The migration of BMSCs could be remarkably stimulated by appropriate cyclic stretch, and the number of cells was increased to 1.58 times compared with that of the control group at 1 Hz, 10% strain for 8 hours. Moreover, the expression level of matrix metallopoteinases-2, -9 protein was increased signific antly after the stimulation of the stretch. After treated with GM6001, the secretion of MMP-2,-9 and the migration of BMSCs stimulated by the cyclic stretch was inhibited prominently. Conclusions: Matrix metallopoteinases-2, -9 play an important role in the cours that the cyclic stretch regulated on the migration of BMSCs.
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Received: 13 January 2012
Published: 25 May 2012
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