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| Effects of carAB and pyrBI Overexpression on Cytidine Fermentation in Escherichia coli |
| WU Xiao-jiao, SUN Jia-kai, HUO Wen-ting, XIE Xi-xian, XU Qing-yang, CHEN Ning |
| Key Laboratory of Industrial Microbiology, Ministry of Education and Tianjin City, School of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China |
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Abstract To study the effects of carbamoyl phosphate synthetase and aspartate carbamoyl transferase on cytidine fermentation of E. coli, carAB and pyrBI were amplified by PCR from E. coli A39 (△cdd) chromosome and ligated into the pSTV28 vector. Then the recombinant plasmids pSTV28-carAB and pSTV28-pyrBI were transformed into E. coli A39 (△cdd). And the effects of pSTV28-carAB and pSTV28-pyrBI on cell growth, cytidine and uridine production and accumulation of acetic acid were studied by fermentation experiments. The results suggested that cytidine productions of E. coli A39-AB and A39-BI were 583.5 mg/L and 408.4 mg/L, which were increased by 85.3% and 29.7% compared with A39 (△cdd). It shows that Overexpressing carAB and pyrBI operon can promote the accumulation of cytidine.
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Received: 14 November 2011
Published: 25 February 2012
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