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| Spongelike Small Intestinal Submucosa Matrix Can Promote Osteoblast-like Cells Proliferation and Differentiation |
| FANG Yan1, NI Wei-min2, SHAN Wei1, ZENG Rui-xia1, LIU Xue-yuan1 |
1. Department of Anatomy of Basic Medical College of LiaoNing Medical University, Jinzhou 121000, China;
2. Department of Neurosurgery of the First Affiliated Hospital of LiaoNing Medical University, Jinzhou 121000, China |
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Abstract Objective: Observe the growth of osteoblast-like cells induced and differentiated by human adipose stem cells (hADSCs) in the spongelike porcine small intestinal matrix (SIS). Explore ability of three-dimensional SIS promote osteoblast-like cells proliferation and differentiation. Methods: acellular SIS is prepared by combination of physical and chemical methods with porcine proximal jejunum. Particles was made with film-like SIS by milling-in liquid nitrogen cryogenic grinding. And the particles has been reshaped spongelike after crosslinked by freeze-drying technology. hADSCs is isolated and cultured by enzymatic digestion. And surface antigen has been identificated by flow cytometry. hADSCs has been induced and differentiated into osteoblast-like cells、chondroblast-like cells and adipocyte-like cells. The osteoblast-like cells is cultured in cavernous SIS. Cell morpholog is observed by scanning electron microscope. Osteoblast-like cells is cultured in material extracts of SIS. The cell viability is evaluated with MTT. Osteogenic differentiation is detected by ALP activity. Results: Spongelike SIS is three-dimensional stereo-scaffold with a great of regular three-dimensional pore. Stem cell-associated antigen is expressed by primary hADSCs. hADSCs can differentiated into osteoblast-like cells stained by alizarin red. Osteoblast-like cells can proliferate and express ALP obviously in cavernous SIS. Conclusion: spongelike SIS with three-dimensional pores presents superior cytocompatibility, and it can promote osteoblast-like cell differentiated by hADSCs proliferate and increase osteogenic activity significantly. It can became a novel three-dimensional natural biological materials for bone tissue engineering.
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Received: 29 January 2013
Published: 25 June 2013
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| [1] Hoganson D M, Owens G E, O’Doherty E M, et al.Preserved extracellular matrix components and retained biological activity in decellularized porcine mesothelium.Biomaterials, 2010, 31(27):6934-6940.
[2] Roeder R A, Lantz G C, Geddes L A. Mechanical remodeling of small intestine submucosa small-diameter vascular grafts-a preliminary report. Biomed Instrum Technol, 2001,35(2):110-120.
[3] Greca F H, Noronha L, Bendhack M, et al. Use of small intestine submucosa as ureteral allograft in pigs. Int Braz J Urol,2004,30(4):327-334.
[4] Ayyildiz A, Akgül K T, Huri E, et al.Use of porcine small intestinal submucosa in bladder augmentation in rabbit: long-term histological outcome. ANZ J Surg,2008,78(1-2):82-86.
[5] Keskin M, Kelly C P, Moreira-Gonzalez A, et al. Repairing critical-sized rat calvarial defects with a periosteal cell-seeded small intestinal submucosal layer.Plast Reconstr Surg, 2008,122(2):400-409.
[6] Abraham G A, Murray J, Billiar K, et al. Evaluation of the porcine intestinal collagen layer as a biomaterial. J Biomed Mater Res, 2000,51(3):442-452.
[7] Zuk P A, Zhu M, Mizuno H, et al.Multilineage cells from human adipose tissue: implications for cell-based therapies.Tissue Eng,2001,7(2):211-226.
[8] Tang L, Yin Y, Zhou H, et al. Proliferative capacity and pluripotent characteristics of porcine adult stem cells derived from adipose tissue and bone marrow.Cell Reprogram,2012,14(4):342-352.
[9] Pan Feng, Bai Shuling. The experimental research on cultivation and differentiation of rabbit BMSCs in vitro. Jie Pou Ke Xue Jin Zhan, 2005, 11 (1): 12-15.
[10] Lawrence B J, Maase E L, Lin H K, et al.Multilayer composite scaffolds with mechanical properties similar to small intestinal submucosa.J Biomed Mater Res A, 2009,88(3):634-643.
[11] Yang B, Zhou L, Sun Z, et al.In vitro evaluation of the bioactive factors preserved in porcine small intestinal submucosa through cellular biological approaches.J Biomed Mater Res A,2010, 93(3):1100-1109. |
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