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| Progress in the Application of Non-immunoglobulin Scaffolds |
| LI Geng, LIU Xiao-zhi, WANG Zhi-ming, GAO Jian |
| State Key Laboratory of Antibody Research & Development;New Drug Research and Development Company Ltd., North China Pharmaceutical Corporation, Shijiazhuang 050015, China |
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Abstract Compared with the antibody drugs, the non-immune globulin scaffolds with a small molecular weight, do not need to be modified after translation, usually lack disulfide bonds, and can undergo straightforward multimerization. In recent years, the development of non-immune globulin scaffolds have reached more than 20 different types, such as Adhirons, Alphabodies, Centyrins, Pronectins, Repebodies, Affimers, and Obodies. 102 proteins have been specifically targeted by 139 different non-Ig scaffold binders. These non-immune globulin scaffolds are used for the treatment and diagnosis of cancer and inflammatory diseases, and there are more than 10 types of non-immune globulin scaffolds have been used in clinical trials. Recently, non-immune globulin scaffolds have also been used in research as structure determination chaperones, which are used in the study for intracellular monitoring of post-translational modifications or as an alternative to antibodiesfor microscopy, flow cytometry, and Western blotting, and so on.
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Received: 11 September 2015
Published: 19 November 2015
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| [1] Koerber J T,Hornsby M J,Wells J A.An improved single-chain Fab platform for efficient display and recombinant expression.Journal of Molecular Biology,2015,427(2):576-586.
[2] Skrlec K,Strukelj B,Berlec A.Non-immunoglobulin scaffolds:a focus on their targets.Trends in Biotechnology,2015,33(7):408-418.
[3] Levisson M,Spruijt R B,Winkel I N,et al.Phage display of engineered binding proteins.Methods in Molecular Biology,2014,1129:211-229.
[4] Lee Y,Lee J J,Kim S,et al.Dissecting the critical factors for thermodynamic stability of modular proteins using molecular modeling approach.PloS One,2014,9(5):e98243.
[5] Lin H,Zhou J,Shen L,et al.Biotin-conjugated anti-CD44 antibody-avidin binding system for the improvement of chondrocyte adhesion to scaffolds.Journal of Biomedical Materials Research Part A,2014,102(4):1140-1148.
[6] Helma J,Cardoso M C,Muyldermans S,et al.Nanobodies and recombinant binders in cell biology.The Journal of Cell Biology,2015,209(5):633-644.
[7] Zielonka S,Empting M,Grzeschik J,et al.Structural insights and biomedical potential of IgNAR scaffolds from sharks.mAbs,2015,7(1):15-25.
[8] Liang S I,McFarland J M,Rabuka D,et al.A modular approach for assembling aldehyde-tagged proteins on DNA scaffolds.Journal of the American Chemical Society,2014,136(31):10850-10853.
[9] Maltezos A,Platis D,Vlachakis D,et al.Design,synthesis and application of benzyl-sulfonate biomimetic affinity adsorbents for monoclonal antibody purification from transgenic corn.Journal of Molecular Recognition,2014,27(1):19-31. |
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