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A Comparative Study of Three Ways of Acellular Process on Small Intestinal Submucosa's Biocompatibility and Immunogenicity |
HUANG Wei-feng, CHENG Peng, JIANG Ping |
Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China |
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Abstract Objective: To compare the effect of three ways of acellular process on biocompatibility and immunogenicity of the small intestinal submucosa, preparing for the scaffold of tissue engineering skin. Methods: Fresh jejunum of pig was prepared by mechanical method, mechanical-chemical method and machanical-enzymicmethod into SIS, kept as groups A,B,C, respectively. Fibroblasts were seeded on the SIS scaffolds to construct the derm substitute. The comparative examinations were performed by histological observation, MTT assay to observe the structure of the scaffolds, proliferation and adhesion of the cell on the scaffolds. The inflammation cause by the scaffolds after subcutaneous implantation for 1,2,4 weeks were also analysed. Results: Histological observation shows that there were no residual cells in groups B and C, but residual cells in group A. The proliferation and adhesion test indicated that group A was better than the other two(P<0.05).The subcutaneous implantation analysis showed that group B caused a less serious inflammation, and the vascularization capacity of group C was greater than groups A and B. Conclusion: Mechanical-chemical method and machanical-enzymic method were better ways to prepare SIS as a scaffold for tissue engineering skin.
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Received: 11 March 2015
Published: 25 June 2015
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