Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (6): 54-60    DOI: 10.13523/j.cb.20150609
    
A Comparative Study of Three Ways of Acellular Process on Small Intestinal Submucosa's Biocompatibility and Immunogenicity
HUANG Wei-feng, CHENG Peng, JIANG Ping
Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Download: HTML   PDF(1249KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Objective: To compare the effect of three ways of acellular process on biocompatibility and immunogenicity of the small intestinal submucosa, preparing for the scaffold of tissue engineering skin. Methods: Fresh jejunum of pig was prepared by mechanical method, mechanical-chemical method and machanical-enzymicmethod into SIS, kept as groups A,B,C, respectively. Fibroblasts were seeded on the SIS scaffolds to construct the derm substitute. The comparative examinations were performed by histological observation, MTT assay to observe the structure of the scaffolds, proliferation and adhesion of the cell on the scaffolds. The inflammation cause by the scaffolds after subcutaneous implantation for 1,2,4 weeks were also analysed. Results: Histological observation shows that there were no residual cells in groups B and C, but residual cells in group A. The proliferation and adhesion test indicated that group A was better than the other two(P<0.05).The subcutaneous implantation analysis showed that group B caused a less serious inflammation, and the vascularization capacity of group C was greater than groups A and B. Conclusion: Mechanical-chemical method and machanical-enzymic method were better ways to prepare SIS as a scaffold for tissue engineering skin.



Key wordsSmall submucosa intestinal      Acellularization      Fibroblast      Derm substitute      Cell scaffold     
Received: 11 March 2015      Published: 25 June 2015
ZTFLH:  Q813.1  
Cite this article:

HUANG Wei-feng, CHENG Peng, JIANG Ping. A Comparative Study of Three Ways of Acellular Process on Small Intestinal Submucosa's Biocompatibility and Immunogenicity. China Biotechnology, 2015, 35(6): 54-60.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150609     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I6/54


[1] Badylak S F, Lindberg K. Porcine small intestinal submucosa (SIS): a bioscaffold supporting in vitro primary human epidermal cell differentiation and synthesis of basement membrane proteins. Burns,2001,27(3):254-266.

[2] Shi L, Ronfard V. Biochemical and biomechanical characterization of porcine small intestinal submucosa (SIS): a mini review. Int J Burns Trauma,2013,3(4):173-179.

[3] Matsumoto T, Holmes R H, Burdick C O, et al. Replacement of large veins with free inverted segments of small bowel: autografts of submucosal membrane in dogs and clinical use. Ann Surg,1966,164(5):845-848.

[4] Ansaloni L, Cambrini P, Catena F, et al. Immune response to small intestinal submucosa (surgisis) implant in humans: preliminary observations. J Invest Surg,2007,20(4):237-241.

[5] Ashley R A, Roth C C, Palmer B W, et al. Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model. BJU Int,2010,105(10):1462-1468.

[6] Keskin M, Kelly C P, Moreira-Gonzalez A, et al. Repairing critical-sized rat calvarial defects with a periosteal cell-seeded small intestinal submucosal layer. Plast Reconstr Surg,2008,122(2):400-409.

[7] Badylak S, Obermiller J, Geddes L, et al. Extracellular matrix for myocardial repair. Heart Surg Forum,2003,6(2):E20-E26.

[8] Badylak S F, Tullius R, Kokini K, et al. The use of xenogeneic small intestinal submucosa as a biomaterial for Achilles tendon repair in a dog model. J Biomed Mater Res,1995,29(8):977-985.

[9] Badylak S F, Gilbert T W. Immune response to biologic scaffold materials. Semin Immunol,2008,20(2):109-116.

[10] Kalota S J. Small intestinal submucosa tension-free sling: postoperative inflammatory reactions and additional data. J Urol,2004,172(4 Pt 1):1349-1350.

[11] Abraham G A, Murray J, Billiar K, et al. Evaluation of the porcine intestinal collagen layer as a biomaterial. J Biomed Mater Res,2000,51(3):442-452.

[12] 陈薇,李次会,武术,等. 脱细胞处理对小肠黏膜下层细胞残留及生长因子含量影响的实验研究. 中国修复重建外科杂志,2010,24(1):94-99. Chen W, Li C H, Wu S, et al. Effect of acellular process on small intestinal submucosa cell residue and growth factor content. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi,2010,24(1):94-99.

[13] 陈伟,姜平. 活性真皮替代物的体内外实验. 中国组织工程研究,2012,16(51):9607-9610. Chen W, Jian P. In vivo and in vitro experiment of living derm substitute. Zhongguo Zuzhi Gongcheng Yanjiu,2012,16(51):9607-9610.

[14] Burugapalli K, Pandit A. Characterization of tissue response and in vivo degradation of cholecyst-derived extracellular matrix. Biomacromolecules,2007,8(11):3439-3451.

[15] Andree B, Bar A, Haverich A, et al. Small intestinal submucosa segments as matrix for tissue engineering: review. Tissue Eng Part B Rev,2013,19(4):279-291.

[16] Badylak S F, Taylor D, Uygun K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng,2011,13:27-53.

[17] Hodde J P, Record R D, Liang H A, et al. Vascular endothelial growth factor in porcine-derived extracellular matrix. Endothelium,2001,8(1):11-24.

[18] Voytik-Harbin S L, Brightman A O, Kraine M R, et al. Identification of extractable growth factors from small intestinal submucosa. J Cell Biochem,1997,67(4):478-491.

[19] Gilbert T W, Sellaro T L, Badylak S F. Decellularization of tissues and organs. Biomaterials,2006,27(19):3675-3683.

[20] Boughner D R, Cimini M, Ronald J A, et al. Dermal fibroblasts cultured on small intestinal submucosa: Conditions for the formation of a neotissue. J Biomed Mater Res A,2005,75(4):895-906.

[21] Liu S, Zhang H, Zhang X, et al. Synergistic angiogenesis promoting effects of extracellular matrix scaffolds and adipose-derived stem cells during wound repair. Tissue Engineering Part A,2011,17(5-6):725-739.

[22] Mostow E N, Haraway G D, Dalsing M, et al. Effectiveness of an extracellular matrix graft (OASIS Wound Matrix) in the treatment of chronic leg ulcers: a randomized clinical trial. J Vasc Surg,2005,41(5):837-843.

[23] Romanelli M, Dini V, Bertone M S. Randomized comparison of OASIS wound matrix versus moist wound dressing in the treatment of difficult-to-heal wounds of mixed arterial/venous etiology. Adv Skin Wound Care,2010,23(1):34-38.

[24] Romanelli M, Dini V, Bertone M, et al. OASIS wound matrix versus Hyaloskin in the treatment of difficult-to-heal wounds of mixed arterial/venous aetiology. Int Wound J,2007,4(1):3-7.

[25] Shi L, Ramsay S, Ermis R, et al. In vitro and in vivo studies on matrix metalloproteinases interacting with small intestine submucosa wound matrix. Int Wound J,2012,9(1):44-53.

[26] Zhou Y, Yan Z, Zhang H, et al. Expansion and delivery of adipose-derived mesenchymal stem cells on three microcarriers for soft tissue regeneration. Tissue Eng Part A,2011,17(23-24):2981-2997.

[27] Kim S H, Song J E, Lee D, et al. Development of poly(lactide-co-glycolide) scaffold-impregnated small intestinal submucosa with pores that stimulate extracellular matrix production in disc regeneration. J Tissue Eng Regen Med,2014,8(4):279-290.

[1] WANG Yuan-dou,SU Feng,LI Su-ming. Research Progress of Photocrosslinked Hydrogel in Tissue Engineering[J]. China Biotechnology, 2020, 40(4): 91-96.
[2] ZHENG Jie, JIANG Chao, LI Xiao-kun, TIAN Hai-shan. The Progression of Fibroblast Growth Factor 6[J]. China Biotechnology, 2017, 37(4): 110-114.
[3] GONG Wei-yue, TIAN Hai-shan, LI Xiao-kun, JIANG Chao. Fibroblast Growth Factor and Bone Related Diseases[J]. China Biotechnology, 2016, 36(8): 99-104.
[4] DENG Chun-pin, YANG Bo, MEI Xiong, ZHENG Zan-shun, QU Wei. Measurement and Analysis of Recombinant Basic Fibroblast Growth Factor's Free Sulfhydryl[J]. China Biotechnology, 2016, 36(6): 76-80.
[5] YI Shan-yong, YANG Jing, GUAN Li-li, WANG Yan-fang, HUANG Jian, WANG Li-yong, LI Hai-yan, LI Xiao-kun, JIANG Chao. Research Progresses On The Fibroblast Growth Factor 9[J]. China Biotechnology, 2015, 35(7): 94-101.
[6] ZHANG Chao, XIANG Li-na, CHEN De-pei, LÜ Xin-xin, ZHAO Yi-tong, WANG Lu-yao, XIAO Jian, ZHANG Hong-yu. The Development of the Study on bFGF Promote the Nerve Injury Repair[J]. China Biotechnology, 2015, 35(6): 75-79.
[7] LUO Chan, GONG Yun, REN Yan-ping, YANG Su-fang, RUAN Qiu-yan, GUAN Xiao-mei, JIANG Jian-rong, SHI De-shun. Optimization of Electroporation Condition for Buffalo Fetal Fibroblast Cells[J]. China Biotechnology, 2013, 33(9): 59-65.
[8] HUANG Peng-huang, WANG Ze, TIAN Hai-shan, ZHAO Hai-yang, LI Hai-yan, LI Xiao-kun. The Constructing and Purification of Recombinant Human Fibroblast Growth Factor 8b Expressed Vector[J]. China Biotechnology, 2013, 33(1): 14-19.
[9] WANG Yi, TIAN Hai-shan, LI Xiao-kun. The Development of Fibroblast Growth Factor 8[J]. China Biotechnology, 2011, 31(01): 75-80.
[10] LIU Guan-Lan, LI Tian, LIU Jin-Yuan, YAN Ze-Min, DUAN Meng-Xing. Protection Effects of Prokaryotic Expressed Radish Phospholipid Hydroperoxide Glutathione Peroxidase and Glutathione on Hydroperoxidemediated Injury in Mouse NIH3T3 Fibroblasts[J]. China Biotechnology, 2010, 30(09): 13-18.
[11] LIN Jian-Cong, ZHANG Min-Jing, SU Zhi-Jian, CHEN Gong-Xia, QIU Zhuang-Wei, LOU Guo-Feng, XIANG Qi, HUANG E-Dong. Expression, Purification and Bioassay of Tat-aFGF Fusion Protein in Escherichia coli[J]. China Biotechnology, 2010, 30(05): 11-17.
[12] CHU Pan-Hui, LIU Hai-Feng, WANG Xiao-Hua, ZHANG Hu-Fei, WU Yan, YUAN Xiao-Huan. Expression and Characterization of a Novel Truncated TGF-&beta|Receptor II Synthesized in Escherichia coli[J]. China Biotechnology, 2009, 29(08): 33-37.
[13] . Activity study of specific bFGF-binding phage[J]. China Biotechnology, 2009, 29(01): 23-26.
[14] . Selection of bFGF mimic peptide by phage display[J]. China Biotechnology, 2006, 26(05): 7-10.