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| The Anti-angiogenesis Activity of Mycobacterium Tuberculosis Heat Shock Protein 65 in Intradermal B16-F10 Melanoma-bearing Mice |
| XIE Yan-fei1,2, CHEN Ying-ying2, ZUO Ai-ren1, CAO Rong-yue2 |
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;
2. Minegene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China |
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Abstract To observe the anti-angiogenesis activity of Mycobacterium Tuberculosis Heat Shock Protein 65, a prokaryotic expression vector pET-28a-Hsp65 was built. The recombination protein HSP65 were acquired by anion exchange column chromatography and then mixed with Freund's adjuvant to immunize the C57BL/6J mice once a week for six times. Two weeks after last immunization, B16-F10 melanomas cells were intradermally inoculated to investigate the immunogenicity and pharmacodynamic action of these vaccines. The results showed that the persistently high titer of anti-HSP65 antibodies were induced and the angiogenesis around the tumor were significantly inhibited by 41.01% compared with the control group. To further explore the possible mechanism, immunohistochemistry were done to detect the VEGF expression in tumor surface which showed a significant reduction in immunized group. However, the precise mechanism still need more investigation.
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Received: 11 July 2012
Published: 25 November 2012
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