<i>Lactobacillus plantarum</i> Exopolysaccharide Coated High-Stable Selenium Nanoparticles and Its Antioxidant Activity

doi:10.13523/j.cb.2005056

China Biotechnology

2020, Vol. 40

Issue (9): 18-27 DOI: 10.13523/j.cb.2005056

Lactobacillus plantarum Exopolysaccharide Coated High-Stable Selenium Nanoparticles and Its Antioxidant Activity

CHEN Dong,LI Cheng-cheng,SHI Zhong-ping(

)

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

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Abstract

Objective: The highly stable EPS-nano-selenium complexes (E-SeNPs) were safely and simply prepared using Lactobacillus plantarum exopolysaccharides (EPS) as stabilizers and coating agents. The stability and antioxidant activity were also studied. Methods: The size, morphology, structure and stability of E-SeNPs were measured by transmission electron microscopy (TEM), dynamic light scattering (DLS), ultraviolet-visible spectroscopy (UV-vis), and Fourier transform infrared spectroscopy (FT-IR). In addition, the antioxidant activity of E-SeNPs in vitro was evaluated by measuring the reducing ability and the clearance rate of ABTS⁺. Results: E-SeNPs with good dispersibility and stability were successfully prepared. The average particle size of the prepared E-SeNPs is (45.17±11.9)nm, with a negative charge (-31.3mV). Meanwhile, the E-SeNPs can be stable in the aqueous solution for 20 days. It was found by FT-IR analysis that the stability was due to the coating effect of EPS. Finally, at the same concentration, the reducing power and ABTS⁺ radical scavenging rate of E-SeNPs were significantly higher than those of EPS and selenium nanoparticles (SeNPs), showing good antioxidant activity. Conclusion: A new type of SeNPs stabilizer and coating agents were developed. It was simple and safe to prepare highly stable, water-dispersible SeNPs with good antioxidant activity.

Key words： Lactobacillus plantarum Exopolysaccharide Selenium nanoparticles Stability Antioxidant activity

Received: 25 May 2020 Published: 12 October 2020

ZTFLH:

Q819

Corresponding Authors: Zhong-ping SHI E-mail: zpshi@jiangnan.edu.cn

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	Dong CHEN
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Cite this article:

CHEN Dong,LI Cheng-cheng,SHI Zhong-ping. Lactobacillus plantarum Exopolysaccharide Coated High-Stable Selenium Nanoparticles and Its Antioxidant Activity. China Biotechnology, 2020, 40(9): 18-27.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2005056 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I9/18

Fig.1 Schematic illustration for isolation, screening and identification of Lactobacillus plantarum (a)Fuyuan pickles (b)Colony pictures (c)Wiredrawing test (d)Yield of EPS from different strains (e)Gram staining of Lactobacillus plantarum

Fig.2 Pictures of selenium nanoparticles synthesized under different ratios

Fig.3 Uv-vis absorption spectra of E-SeNPs synthesized at different ratios

Fig.4 Size distribution of E-SeNPs synthesized at different ratios

Fig.5 Characterization of SeNPs, E-SeNP, EPS-R040 (a) FT-IR spectra of SeNPs, E-SeNP, and EPS-R040 (b) Zeta potential results of SeNPs, E-SeNP, EPS-R040

Fig.6 TEM results of E-SeNPs and SeNPs at different magnifications; the size statistical analysis and distributions of E-SeNPs (a),(b)Morphological structures of E-SeNPs observed by TEM (c),(d)Morphological structures of SeNPs observed by TEM (e),(f)Size distributions of E-SeNPs analyzed by TEM and DLS

Fig.7 The digital photographs of selenium nanoparticles stored at 4℃ for different days Left: Se:EPS=1:0; Right: Se:EPS=4:3 (a)-(f) The digital photographs of selenium nanoparticles stored at 4℃ for 0, 5, 10, 15, 20, and 23 days

Fig.8 The reductive activity of E-SeNPs synthesized at different ratios

Fig.9 Scavenging effect of the E-SeNPs synthesized at different ratios on ABTS⁺


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