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Agrobacterium-mediated Transformation and the Functions of Pks in Marine-derived Penicillium oxalicum |
MENG Xiao-lin,PANG Xi-ming,WANG Jie() |
College of Food Science, South China Agricultural University, Guangzhou 510642, China |
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Abstract To investigate the functions of polyketide synthases (PKS) in Penicillium oxalicum SCSGAF0023, a marine gorgonian coral-associated fungus, the method of Agrobacterium-mediated transformation in P. oxalicum SCSGAF0023 was constructed and used to obtain the disruption mutants of Pks by homologous recombination. The roles of Pks in P. oxalicum SCSGAF0023 were characterized by multi-phenotypic analyses under normal and stressful conditions. An efficient genetic transformation system of P. oxalicum SCSGAF0023 was established under the condition of p0380-hygB being binary vector and conidia being the recipient. The transformation efficiency was the highest when 107 conidia/ml was co-cultivated with Agrobacterium tumefaciens AGL-1 with the OD600 of 0.5 under the induction of 200μmol/L acetoyringone (AS) at 25℃. Based on the Agrobacterium-mediated transformation, the disruption mutants of Pks were obtained, and it was the first reported that Pks positively regulated the conidiation but did not affect the environmental adaptation in P. oxalicum SCSGAF0023, which will be helpful for further investigation on the relationships between PKSs and fungal growth, development and environmental adaptation.
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Received: 22 April 2020
Published: 12 October 2020
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Corresponding Authors:
Jie WANG
E-mail: wangjielangjing@126.com
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