Immunophenotypic Characterization of Nodal Follicular Helper T Cell Lymphoma, Angioimmunoblastic-type

doi:10.13523/j.cb.2310049

China Biotechnology

2023, Vol. 43

Issue (12): 39-45 DOI: 10.13523/j.cb.2310049

Immunophenotypic Characterization of Nodal Follicular Helper T Cell Lymphoma, Angioimmunoblastic-type

WANG Chun-yan¹,LIU Song-ya¹,ZHU Li¹,YI Shu-juan¹,KUANG Dong²,XIAO Ming¹,MAO Xia^1,^*(

)

1 Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Immunotherapy Research Center for Hematologic Diseases of Hubei Province, Wuhan 430030, China
2 Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

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Abstract

Objective: To investigate the immunophenotypic characteristics of nodal follicular helper T-cell lymphoma, angioimmunoblastic-type (nTFHL-A), and to assess the diagnostic value of flow cytometry in nTFHL-A. Methods: A total of 227 patients were included in our study. They were initially diagnosed with nTFHL-A in the Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China, from January 2010 to August 2023. Results: The lack and decreased expression of surface CD3 and the CD7 deficiency were the most common immunophenotypeic abnormalities of pan-T cell markers in nTFHL-A, and CD10 expression was the most specific for nTFHL-A. Almost all neoplastic T cells had bright PD-1 expression and CD45RO expression. Flow cytometry may present false negative results in patients with CD3-positive or slightly decreased expression and meantime with CD7-positive or partial expression (tissue 11.3%, bone marrow 10.3%). Bright PD-1 expression can improve nTFHL-A detection and also help to differentiate it from other peripheral T-cell lymphomas. Conclusions: Flow cytometry is an effective tool to screen, identify or differentiate nTFHL-A.

Key words： T-cell lymphoma Flow cytometry Programmed cell death 1 (PD-1) Immunophenotype

Received: 09 October 2023 Published: 16 January 2024

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	Chun-yan WANG
	Song-ya LIU
	Li ZHU
	Shu-juan YI
	Dong KUANG
	Ming XIAO
	Xia MAO

Cite this article:

Chun-yan WANG, Song-ya LIU, Li ZHU, Shu-juan YI, Dong KUANG, Ming XIAO, Xia MAO. Immunophenotypic Characterization of Nodal Follicular Helper T Cell Lymphoma, Angioimmunoblastic-type. China Biotechnology, 2023, 43(12): 39-45.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2310049 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I12/39

Fig.1 The mean fluorescent intensity of PD-1 in nonneoplastic CD4⁺ T cells and neoplastic T cells A.PD-1 mean fluorescent intensity (MFI) of neoplastic T cells(red dots) in nTFHL-A is significantly higher than that in corresponding nonneoplastic CD4⁺ T cells(blue dots) in different specimens(P<0.001) B. PD-1 MFI of neoplastic T cells(red dots) in nTFHL-A is significantly higher than that in other T-cell lymphomas (blue dots) in different specimens(P<0.05). ROC curve of PD-1 MFI in bone marrow and tissue specimens shows high sensitivity and specificity in differentiating nTFHL-A from other T-cell lymphomas(P<0.001, AUC 0.959: sensitivity 81.3% and specificity 94.6% for a cut-off of MFI 1 183 in BM samples; P<0.001, AUC 0.979: sensitivity 92.3% and specificity 95.6% for a cut-off of MFI 2 023 in tissue samples)

Fig.2 The features of PD-1 expression in different T-cell lymphomas and different subgroups A. The features of PD-1 expression in different T-cell lymphomas (tissue samples). a:Neoplastic T cells of nTFHL-A had bright PD-1 expression;b:PD-1 is negative to moderate intensity in other PTCLs; c-f: PD-1 is usually negative in anaplastic large cell lymphoma(c),sézary syndrome(d),mycosis fungoides(e) and lymphocytic-variant hypereosinophilic syndromes(f); g: Polyclonal CD4⁺PD-1^bri+ T cells could be observed in nodular lymphocyte predominant Hodgkin lymphoma; h: Polyclonal CD4⁺PD-1^bri+ T cells could be observed in reactive lymphoid hyperplasia B.The features of PD-1 expression in different subgroups in nTFHL-A. TRBC-1 is monoclonal in CD4⁺PD-1^bri+ neoplastic T cells;TRBC1 is polyclonal in CD4⁺PD-1⁺ and CD4⁺PD-1^- normal T cells

Table 1 The expression of other non-pan-T cell antigens in nTFHL-A


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