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| Exploring the Nanoworld: Development and Single-Particle-Level Characterization of Nano-flow Cytometry Technology |
CAI Nian-gui,CHEN Xin,ZHANG Qing-yuan,DI Hao-nan,ZHAN Xiao-zhen,CHEN Jun-yan,CHEN Hao,YAN Xiao-mei( ) |
| Key Laboratory of Spectrochemical Analysis &Instrumentation, Ministry of Education, Fujian Provincial Key Laboratory of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China |
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Abstract Accurate characterization of nanoparticles is of paramount importance for advancing our understanding of life sciences, improving disease diagnosis and treatment, and fostering the development of nanotechnology. Due to the high degree of individual variation and heterogeneity among nanoparticles, there is an urgent need for the development of rapid detection techniques at the single-particle level. This review article introduces the development of nano-flow cytometry (nFCM), a technology based on Rayleigh scattering and sheath-flow single-molecule fluorescence detection. nFCM can achieve highly sensitive, selective, and high-throughput multi-parameter analysis of the size distribution, particle concentration, and biochemical characteristics of synthetic nanoparticles (7 ~ 500 nm), as well as naturally occurring biological nanoparticles such as extracellular vesicles and viruses, at a rate of up to 10 000 particles per minute. The article discusses the significance, challenges, progress, and industrial transformation of the nFCM development, reviews its applications in nanoparticle research, and discusses its future application prospects.
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Received: 09 October 2023
Published: 16 January 2024
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