Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province

Yi-hua HUI; Hai-na WANG; Yu-feng QI; Xue-ling CAO; Xue-mei GUAN; Jing-jing DUAN; Yi- jun DUAN; Yan- feng WANG; Wen SU

doi:10.13523/j.cb.20190906

PDF(1366 KB)

China Biotechnology ›› 2019, Vol. 39 ›› Issue (9) : 41-49. DOI: 10.13523/j.cb.20190906

Orginal Article

Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province

Author information +

History +

Abstract

Objective: To establish normal reference range of peripheral blood lymphocyte subsets in healthy adults in Shanxi Province and provide theoretical basis for studying immunity status and assessing immune function in cancer patients.Methods: Selected 1 238 healthy adults in Shanxi Province and used six-color monoclonal antibody to detect and analysis absolute and percentage counts of peripheral blood lymphocyte subsets.Results: The expression of peripheral blood lymphocytes levels was determined, and it was found that CD3 ⁺T、CD4 ⁺T、CD8 ⁺T、NK、CD19 cell percentage and absolute counts and CD4/CD8 ratio dissimilarity with a P value <0.05 was considered statistically significant ;and there was no statistical significance in CD8 ⁺ T cell percentage counts、CD4 ⁺T cell and CD19 cell absolute counts between different genders,CD3 ⁺T cell、CD8 ⁺T cell、NK cell percentage and absolute counts、CD4 ⁺T cell、CD19 cell percentage counts dissimilarity with a P value <0.05 was considered statistically significant. Conclusion: The refe -rence range of peripheral blood lymphocyte subsets in healthy adults in Shanxi Province was preliminarily established ,which provided reference for the evaluation of immunity function, tumor immunotherapy and diagnosis.

Key words

T lymphocyte subsets / Normal reference values / Flow cytometry / ShanXi Province

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Yi-hua HUI, Hai-na WANG, Yu-feng QI, et al. Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province[J]. China Biotechnology, 2019, 39(9): 41-49 https://doi.org/10.13523/j.cb.20190906

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis

[1]	Keishi Adachi, Koji Tamada . Immune checkpoint blockade opens an avenue of cancer immunotherapy with a potent clinical effificacy. Cancer Science, 2015,106(8):945-950. Cited in this article [1]

[2]	Li J, Xu L, Run Z C , et al. Multiple cytokine profiling in serum for early detection of gastric cancer. World J Gastroenterol , 2018,24(21):2269-2278. Cited in this article [1]

[3]	Qouzi A A, Salamah A A, Rasheed R A , et al. Immunophenotyping of peripheral blood lymphocytes in Saudi men. Clinical and Vaccine Immunology, 2002,9(2):279-281. Cited in this article [1]

[4]	Apoil P A, Puissant-Lubrano B, Congy-Jolivet N , et al. Reference values for T, B and NK human lymph-ocyte subpopulations in adults. Data in Brief, 2017,2(1) 400-404. Cited in this article [1]

[5]	Lee B W, Yap H K, Chew F T , et al. Age- and sex-related changes in lymphocyte subpopulations of healthy Asian subjects: from birth to adulthood. Cytometry, 1996,26(1):8-15. Cited in this article [1]

[6]	McCoy J P Jr, Overton W R . Quality control in flow cytometry for diagnostic pathology:II. A conspectus of reference ranges for lymphocyte immunophenotyping. Cytometry, 1994,18(3):129-139. Cited in this article [1]

[7]	Amatya R, Vajpayee M, Kaushik S , et al. Lymphocyte immunophenotype reference ranges in healthy Indian adults: implications for management of HIV/AIDS in India. Clinical Immunology, 2004,112(3):290-295. Cited in this article [1]

[8]	Akgün Y, Salih C, Filiz K , et al. Reference ranges of lymphocyte subsets of healthy adults in Turkey. Med Princ Pract, 2005,14(3):189-193. Cited in this article [3]

[9]	Adoga M P, Pennap G R, John P A , et al. CD4- and CD3-T lymphocyte reference values of immunocompetent urban and rural subjects in an African nation. Scandinavian Journal of Immunology, 2012,76(1):33-38. Cited in this article [1]

[10]	Hidenori S, Eisuke A, Lay A L , et al. CD4/CD8 ratio predicts the cellular immune response to acute hepatitis C in HIV-coinfected adults. Journal of Infection and Chemotherapy, 2019,25(8):646-648. Cited in this article [1]

[11]	Bisset L R, Lung T L, Kaelin M , et al. Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland. Eur J Haematol, 2004,72(3):203-212. Cited in this article [4]

[12]	Uppal S S, Verma S, Dhot P S . Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking.Cytometry Part B -Clin Cytom, 2003 , 52(1):32-36. Cited in this article [3]

[13]

Valiathan

, Deeb

, Diamante

, et al. Reference ranges of lymphocyte subsets in health -y adults and adolescents with special mention of T cell maturation subsets in adults of South Florida. Immunobiology, 2014,219(7):487-496.

https://doi.org/10.1016/j.imbio.2014.02.010

Cited in this article [1] Abstract

Background: Analysis of peripheral blood lymphocyte subsets has become an essential tool in the evaluation of outcome of diagnostic and research related questions in immunological and pathological conditions. Periodic evaluation and establishment of normal lymphocyte reference ranges are required in clinical and research settings of various immunodeficiency disorders for evaluation of the significance of observations. It is also important that age and gender specific lymphocyte subset reference ranges should be locally established for meaningful comparison and accurate result interpretation as age plays a significant role in the development of immune system. Methods: We performed dual platform flow cytometry to determine reference ranges for lymphocyte subsets (CD3, CD4, CD8, CD19 [B cells] and CD16+CD56+ [Natural Killer - NK cells]) in 50 adolescents (age range: 12-18) and 100 adults (age range: 21-67) along with T cell maturation, activation and costimulatory molecules in healthy multiracial adult population of South Florida. Results: The lymphocyte reference ranges percentages [absolute counts - Abs, cells/mu l] unadjusted for gender differences for adolescents are: CD3: 49-83 [939-2959]; CD4: 27-53 [467-1563]; CD8: 16-40 [259-1262]; CD19+ B cells: 8-31 [169-1297] and CD16+CD56+ NK cells: 3-30 [59-1178] and for adults are: CD3: 65-88 [983-3572]; CD4: 26-62 [491-2000]; CD8: 14-44 [314-2,087]; CD19+ B cells: 2-27 [64-800] and CD16+CD56+ NK cells: 2-27 [27-693]. The ranges for CD4:CD8 ratio for adolescents and adults are 0.7-2.6 and 0.6-4.4, respectively. Gender based analysis of relative percentages of lymphocyte subsets showed no significant differences between adult and adolescent males and females. The mean CD4:CD8 ratio was significantly higher in adult females than males (P=0.04) and in adolescents this difference was not significant between genders. The mean CD3 and CD4 T cell percentages were higher and CD19 cell percentages were lower in adults compared to adolescents (P<0.0001). Absolute lymphocyte counts showed a positive correlation with the absolute counts of CD3+, CD4+, CD8+, CD19+, CD16+CD56+, CD45RO+ and CD45RA+ cells (all correlations with P < 0.0001 except CD45RO [P = 0.01] and CD45RA [P = 0.03]). Conclusion: The reference values of peripheral blood lymphocyte subsets were analyzed in healthy adolescent and adult population of South Florida. This study indicates the need for periodic evaluation and establishment of lymphocyte reference ranges for patient population served based on gender and age since these could influence immune status and treatment outcome. (C) 2014 Elsevier GmbH. All rights reserved.

[14]	孙大菊, 刘颖男, 吕惠影 , 等. 长春地区120例健康成人外周血T淋巴细胞亚群的参范围. 中国实验诊断学, 2017,21(12):2160-2162. Sun D J, Liu Y N, Lv H Y , et al. Reference range of peripheral T lymphocyte subsets in 120 healthy adults in Changchun area. Chinese Experimental Diagnostics, 2017,21(12):2160-2162. Cited in this article [1]

[15]	Grace R P, Moses P A, Joseph C F , et al. CD4+T lymphocyte reference values of immunocompetent subjects in an African university. Trop Doct, 2011,41(04):218-221. Cited in this article [1]

[16]	Namazi M R . The Th1-promoting effects of dehydroepiandrosterone can provide an explanation for the stronger Th1-immune response of women. Iran J Allergy Asthma Immunol, 2009,8(1):65-69. Cited in this article [1]

[17]	Jentsch-Ullrich K, Koenigsmann M, Mohren M , et al. Lymphocyte subsets’ reference ranges in an age- and gender-balanced population of 100 healthy adults—A monocentric German study. Clin Immunol, 2005,116(2):192-197. Cited in this article [3]

[18]	Kaaba S A, Fadhli Al S, Khamis A , et al. Reference values of lymphocyte subsets in the normal healthy adult Kuwaiti Arab population. Immunol Lett, 2002,81(3):199-203. Cited in this article [1]

[19]	韩呈武, 于雪莹, 王丽娜 , 等. 北京地区健康成人外周血T淋巴细胞亚群的参考范围. 临床检验杂志, 2009,27(5):390-392. Han C W, Yu X Y, Wang L N . Reference range of peripheral T lymphocyte subsets in 120 healthy adults in Beijing area. Journal of Clinical Laboratory, 2009,27(5):390-392. Cited in this article [3]

[20]	Marcos A, Nova E, Montero A , et al. Changes in the immune system are conditioned by nutrition. Eur J Clin Nutr, 2003,57(Suppl 1):S66-S69. Cited in this article [1]

[21]

Alexander

, Alvaro

, Sapey

, et al. Human innate immunosenescence: causes and consequences for immunity in old age. Trends Immunol, 2009,30(7):325-333.

https://doi.org/10.1016/j.it.2009.05.004

Cited in this article [1] Abstract

The past decade has seen an explosion in research focusing on innate immunity. Through a wide range of mechanisms including phagocytosis, intracellular killing and activation of proinflammatory or antiviral cytokine production, the cells of the innate immune system initiate and support adaptive immunity. The effects of aging on innate immune responses remain incompletely understood, particularly in humans. Here we review advances in the study of human immunosenescence in the diverse cells of the innate immune system, including neutrophils, monocytes, macrophages, natural killer and natural killer T (NKT) cells and dendritic cells—with a focus on consequences for the response to infection or vaccination in old age.

[22]	Mocchegiani E, Muzzioli M, Giacconi R , et al. Metallothioneins/PARP-1/IL-6 interplay on natural killer cell activity in elderly: parallelism with nonagenarians and old infected humans. Effect of zinc supply. Mech Ageing Dev, 2003,124(4):459-468. Cited in this article [1]

[23]

Schenkein J

, Park

, Nahm M

, et al. Pneumococcal vaccination in older adults induces antibodies with low opsonic capacity and reduced antibody potency. Vaccine, 2008,26(43):5521-5526.

[ 24 ] Buffa

, Bulati

, Pellicanò

, et al. B cell immunosenescence: different features of naive and memory B cells in elderly. Biogerontology, 2011,12(5):473-483.

Cited in this article [1]

[25]	Senju M, Makiyama K, Hara K , et al. Two-color immunofluorescence and flow cytometric analysis of peripheral blood lymphocyte subsets in Caucasian and Japanese healthy subjects. Jpn J Med, 1991,30(6):509-515. Cited in this article [1]

[26]	蒋卫民, 潘孝彰, 康来仪 , 等. 上海地区成人不同年龄组间CD4、CD8 淋巴细胞计数正常值调查. 中华传染病杂, 2002,20(4):203-205. Jiang W M, Pan X Z, Kang L Y , et al. Normal CD4 and CD8 lymphocyte counts in adults of different age groups in Shanghai. Chinese Journal of Infectious Diseases, 2002,20(4):203-205. Cited in this article [1]

[27]	Qi Y F, Sun J N, Ren L F , et al. Intestinal microbiota is altered in patients with gastric cancer from Shanxi province, China. Dig Dis Sci, 2018,64(5):1193-1203. Cited in this article [1]