Advances in the Study and Application of <i>in vivo</i> Flow Cytometry

doi:10.13523/j.cb.2310063

China Biotechnology

2023, Vol. 43

Issue (12): 14-23 DOI: 10.13523/j.cb.2310063

Advances in the Study and Application of in vivo Flow Cytometry

LIU Yue-meng,DONG Si-han,WEI Xun-bin(

)

Peking University Health Science Center Institute of Medical Technology, Beijing 100191, China

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Abstract

Flow cytometry (FCM) is a widely used single-cell analysis technique in biological research due to its ability to simultaneously and quantitatively analyze and sort cells or biological particles with multiple parameters in a fast linear flow state. Conventional FCM based on fluorescence has been developed for over 50 years and is a mature technology. However, it requires invasive blood sampling, which disrupts the organism’s physiological environment and limits real-time detection. The in vivo flow cytometry (IVFC) is an emerging technology that enables non-invasive detection of cell samples within living organisms. Through the principles of fluorescence excitation, photoacoustic effect, and photothermal effect, various IVFC techniques have been developed. The basic principles and development of different types of IVFC techniques are summarized and compared with the basic parameters, and the research findings on circulating tumor cells and the current research results of novel label-free assays are reviewed with the aim to show the current development trends and application prospects of IVFC.

Key words： In vivo flow cytometry Circulating tumor cells Label-free imaging

Received: 14 October 2023 Published: 16 January 2024

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	Yue-meng LIU
	Si-han DONG
	Xun-bin WEI

Cite this article:

Yue-meng LIU, Si-han DONG, Xun-bin WEI. Advances in the Study and Application of in vivo Flow Cytometry. China Biotechnology, 2023, 43(12): 14-23.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2310063 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I12/14

Fig.1 Schematic of the optical path structure of the first fluorescent in vivo flow cytometer^[12] The beam of the 632.8 nm He-Ne Laser has a cylindrical lens shaping and passes through a mechanical slit. The beam is focused through the microscope objective, and the laser beam size at the focal plane is approximately 5 μm×72 μm. The fluorescence that occurs is collected by the microscope objective, passed through a dichroic mirror and slit, and the fluorescence is detected by a photomultiplier tube.

Fig.2 Principle of photoacoustic IVFC^[10] When a cell passed through the laser beam, acoustic waves are generated. By detecting and analyzing signals of acoustic waves, cell types can be distinguished. Figure reused with permission ? 2011 John Wiley and Sons. [Color figure can be viewed at wileyonlinelibrary.com]

Table 1 Comparison of flow cytometry parameters of different species in vivo

Fig.3 CTC counts exhibited circadian oscillation in mice monitored by fluorescence IVFC^[54] (a-g) Changes in the number of CTCs in response to circadian rhythms in mice and the highest and lowest values of the number of CTCs in mice with different rhythms (h) Differences in signaling during the passage of individual CTC cells and clusters of CTC cells (i) Daily variations in the number of individual CTCs and clusters of CTCs detected in mice in the 12/12 LD condition


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