| Orginal Article |
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| Development, Application and Prospection of Flow Cytometry |
| Hang Hai-ying1,Liu Chun-chun2,Ren Dan-dan1,** |
1 Key Laboratory for Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2 Core Facility for Protein Research Technology, Tsinghua University, Beijing 100084, China |
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Abstract Flow cytometry, which measures cells,particles or aggregates in flow, is able to provide a large number of single-cell measurements with statistical significance within a short time. After more than 70 years of development, it has become an indispensable technology in many fields including biology, medicine and environmental surveillance. This technology was put forward in the 1940s and formed in the 1970s. In the following 4 decades, the detection performance, multi-parameter measurement ability and sorting ability have been significantly improved. Especially its application fields have expanded rapidly from bacteria measurement, environmental microorganism detection, conventional cell function detection to the diagnosis and monitoring of many clinical diseases, and up to the cutting-edge fields of cancer immune mechanism research, immunotherapy and biopharmaceuticals discovery. At present, a series of new technologies and new types of corresponding flow cytometers have been developed. Although their working principles are different more or less from the traditional method, the concept of obtaining large-scale single cell multi-parameter measurements has never changed. Currently the field of flow cytometry is on the edge of a major development and revolution.
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Received: 24 August 2019
Published: 20 September 2019
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Corresponding Authors:
Dan-dan Ren
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