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Advances in Mechanism of Medium-chain Fatty Acid Toxicity and Construction of Tolerant Strains |
LIU Meng-xiao1,2,HAO Xue-yan1,2,HAN Zi-yi1,2,FANG Li-xia1,2,**(),CAO Ying-xiu1,2,**() |
1 School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China 2 Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education),Tianjin 300072,China |
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Abstract As important platform chemicals, medium-chain fatty acids (MCFAs) are widely used in industries such as energy, food and medicine. The production of MCFAs by industrial microbial fermentation provides a green and environmentally-friendly route, but MCFAs can cause membrane damage, cell pH and osmotic pressure imbalance and oxidative stress, resulting in inhibition of cell growth rate and production capacity. Hence, the construction of MCFA-tolerant industrial microbial strains will improve the production efficiency of MCFAs. In this paper, taking industrial microorganisms such as Escherichia coli and Saccharomyces cerevisiae as examples, the toxicity mechanism of MCFAs to microbial cells is first introduced. Second, the relevant research on using rational metabolic engineering methods such as membrane modification and transporter screening to construct MCFA-tolerant strains is reviewed. Meanwhile, the paper reviews the research progress on the use of such methods as adaptive evolution and metabolic flux analysis to systematically mine MCFA-tolerant targets and improve strain tolerance. Finally, the future research directions for improving the tolerance and production capacity of MCFAs in industrial microorganisms are discussed.
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Received: 21 February 2023
Published: 05 September 2023
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