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| The Expression and Analysis of Terpene Synthesis Related Genes in Maize under the Condition of Salt Stress |
| SHI Li-ping1, JI Jing1, WANG Gang1, JIN Chao1, XIE Chao1, DU Xi-long2, GUAN Chung-feng1, ZHANG Lie3, LI Chen1 |
1. School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China;
2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
3. Company of Tianjin Kerun Jinfeng Seed Industry, Tianjin 300384, China |
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Abstract Terpenes are important secondary metabolites that play a vital role in plant growth and development, some of which can be induced in response to biotic and abiotic stresses. The Tianta 5(F1) and its parent lines were treated with 200 mmol/L NaCl when they were at three-leaf stage. The qRT-PCR results showed the expression level of terpene synthase 2(TPS2), terpene synthase 3(TPS3) and geranylgeranyl diphosphate synthase 4(GGPS4) genes were up-regulated then down-regulated of all lines under salt stress, and the expression level of F1 was much higher than its parent lines.The expression level of three genes was positively correlated with the salt resistance of F2.The content and composition of carotenoids, biomass, photosynthetic indicators, content of chlorophyll and free proline were detected. The results showed that the salt tolerance of F1 was significantly higher than the parent lines. In conclusion, the high expression of TPS2, TPS3 and GGPS4 has a certain correlation with the resistance of salt and other abiotic stress of F1.
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Received: 25 February 2016
Published: 25 August 2016
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Cite this article:
SHI Li-ping, JI Jing, WANG Gang, JIN Chao, XIE Chao, DU Xi-long, GUAN Chung-feng, ZHANG Lie, LI Chen. The Expression and Analysis of Terpene Synthesis Related Genes in Maize under the Condition of Salt Stress. China Biotechnology, 2016, 36(8): 31-37.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160805 OR https://manu60.magtech.com.cn/biotech/Y2016/V36/I8/31
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| [1] Yamaguchi T, Blumwald E. Developing salt-tolerant crop plants:challenges and opportunities. Trends in Plant Science, 2005, 10(12):615-620.
[2] Wang W, Vinocur B, Altman A. Plant responses to drought, salinity and extreme temperatures:towards genetic engineering for stress tolerance. Planta, 2003, 218(1):1-14.
[3] Tholl D. Terpene synthases and the regulation, diversity and biological roles of terpene metabolism. Current Opinion in Plant Biology, 2006, 9(3):297-304.
[4] Bohlmann J, Meyer-Gauen G, Croteau R. Plant terpenoid synthases:molecular biology and phylogenetic analysis. Proceedings of the National Academy of Sciences, 1998, 95(8):4126-4133.
[5] Laskaris G, Bounkhay M, Theodoridis G, et al. Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate. Plant Science, 1999, 147(1):1-8.
[6] Chen F, Tholl D, Bohlmann J, et al. The family of terpene synthases in plants:a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom. The Plant Journal, 2011, 66(1):212-229.
[7] Dudareva N, Martin D, Kish C M, et al. (E)-β-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon:function and expression of three terpene synthase genes of a new terpene synthase subfamily. The Plant Cell, 2003, 15(5):1227-1241.
[8] Taniguchi S, Miyoshi S, Tamaoki D, et al. Isolation of jasmonate-induced sesquiterpene synthase of rice:product of which has an antifungal activity against Magnaporthe oryzae. Journal of Plant Physiology, 2014, 171(8):625-632.
[9] Kappers I F, Aharoni A, Van Herpen T W, et al. Genetic engineering of terpenoid metabolism attracts bodyguards to Arabidopsis. Science, 2005, 309(5743):2070-2072.
[10] Schnee C, K? llner T G, Held M, et al. The products of a single maize sesquiterpene synthaseform a volatile defense signal that attracts natural enemies of maize herbivores. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(4):1129-1134.
[11] Jenkins G I. Signal transduction in responses to UV-B radiation. Annual Review of Plant Biology, 2009, 60:407-431.
[12] Esnault M A, Legue F, Chenal C. Ionizing radiation:advances in plant response. Environmental and Experimental Botany, 2010, 68(3):231-237.
[13] Grote R, Mayrhofer S, Fischbach R J, et al. Process-based modelling of isoprenoid emissions from evergreen leaves of Quercus ilex (L.). Atmospheric Environment, 2006, 40:152-165.
[14] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 2001, 25(4):402-408.
[15] Wintermans J, De Mots A. Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol. Biochimica et Biophysica Acta (BBA)-Biophysics including Photosynthesis, 1965, 109(2):448-453.
[16] Bates L, Waldren R, Teare I. Rapid determination of free proline for water-stress studies. Plant and Soil, 1973, 39(1):205-207.
[17] Wu W,Ji J,Wang G.et al.Overexpression of AtchyB in Eustoma grandiflorum shinn enhances its tolerance to high light via zeaxanthin accumulation.Plant Molecular Biology Reporter,2012,30(6):1433-1444.
[18] Aro E M, McCaffery S, Anderson J M. Photoinhibition and D1 protein degradation in peas acclimated to different growth irradiances. Plant Physiology, 1993, 103(3):835-843.
[19] Ji J,Wang G,Wang J,et al.Functional analysis of multiple carotenogenic genes from Lycium barbarum and Gentiana lutea L for their effects on β-carotene production in transgenic tobacoo.Biotechnology Letters,2009,31(2):305-312.
[20] Lee G W, Lee S, Chung M S, et al. Rice terpene synthase 20(OsTPS20) plays an important role in producing terpene volatiles in response to abiotic stresses. Protoplasma, 2015, 252(4):997-1007.
[21] Rao S A, Mcneilly T. Genetic basis of variation for salt tolerance in maize (Zea mays L). Euphytica, 1999, 108(108):145-150.
[22] Owen S M, Pe?uelas J. Opportunistic emissions of volatile isoprenoids. Trends in Plant Science, 2005, 10(9):420-426.
[23] Zhao Q, Wang G, Ji J, et al. Over-expression of Arabidopsis thaliana β-carotene hydroxylase (chyB) gene enhances drought tolerance in transgenic tobacco. Journal of Plant Biochemistry and Biotechnology, 2014, 23(2):190-198.
[24] Sharkey T D, Yeh S. Isoprene emission from plants. Annual Review of Plant Biology, 2001, 52(1):407-436.
[25] Sharkey T D. Effects of moderate heat stress on photosynthesis:importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Plant Cell and Environment, 2005, 28(3):269-277. |
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