Preparation of Enrofloxacin Molecular Imprinting Electro- chemical Sensor and Its Application to Rapid Detection of Foods

doi:10.13523/j.cb.20190309

China Biotechnology

2019, Vol. 39

Issue (3): 65-74 DOI: 10.13523/j.cb.20190309

Preparation of Enrofloxacin Molecular Imprinting Electro- chemical Sensor and Its Application to Rapid Detection of Foods

Si-nan QIN,Lu-hua TANG,Wen-hui GAO(

)

College of Biological Science and Engineering, Hebei University of Science and Technology, Research Center for Fermentation Engineering of Hebei, Shijiazhuang 050000, China

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Abstract

A novel molecular imprinted electrochemical sensor was prepared by electro-polymerization on the surface of a glassy carbon electrode in NaAc-HAc buffer solution, and enrofloxacin was used as template molecular, o-phenylenediamine (OPD) and o-aminophenol (OAP) as composite functional monomer. The sensor could specifically recognize the template molecules and its analogues. In the experiment, 1 mol/L KCl and 5 mmol/L K₃[Fe(CN)₆] was used as characterization solution, the electrochemical sensor response characteristics were researched, and the preparation and detection conditions of the sensor were optimized by square wave voltammetry (SWV) and cyclic voltammetry (CV). The results showed that under the optimal conditions, the linear relationship between SWV peak current difference and the concentrations of enrofloxacin was found in the range of 2×10 ^-6 mol/L4×10 ^-5 mol/L with the linear correlation coefficient ( R ² ) of 0.9990, the detection limit was 7.0×10 ^-7mol/L, and the sensor had good stability, repeatability and good selectivity to enrofloxacin and its analogues. Enrofloxacin in real samples, such as milk, egg, chicken and pork, was detected by the developed sensor, the average recoveries were between 83.2% and 92.7%, and the relative standard deviations ( RSDs ) were 1.0%~4.8% ( n=5 ). This sensor is simple, sensitive, low cost, and has good selectivity and stability, which provide it a good application prospect.

Key words： Enrofloxacin Food Molecular imprinting polymer Electrochemical sensor Rapid detection

Received: 21 September 2018 Published: 12 April 2019

ZTFLH:

Q0631.3

Corresponding Authors: Wen-hui GAO E-mail: wenhuigao@126.com

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Cite this article:

Si-nan QIN,Lu-hua TANG,Wen-hui GAO. Preparation of Enrofloxacin Molecular Imprinting Electro- chemical Sensor and Its Application to Rapid Detection of Foods. China Biotechnology, 2019, 39(3): 65-74.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190309 OR https://manu60.magtech.com.cn/biotech/Y2019/V39/I3/65

Fig.1 Enrofloxacin molecular structure

Fig.2 CV curves of enrofloxacin in sodium acetate buffer (pH = 5.2)

Fig.3 Electropolymerization curves of 15 mmol/L OPD/OAP in sodium acetate buffer (pH=5.2) (a) Template-free molecules ENRO (b) Template molecules ENRO

Fig.4 Electropolymerization curves of functional monomers in sodium acetate buffer (pH = 5.2) (a) OPD (b) OAP (c) RC (d) OPD/RC (e)0AP/RC

Fig.5 The optimization curve of polymerization ratio

Fig.6 Comparison of the polymerization of template molecule and functional monomer at the same concentration and ratio in PBS electrolyte (pH = 7.2) and NaAc-HAc electrolyte (pH = 5.2) (a) PBS(pH=7.2) (b) NaAc-HAc(pH=5.2)

Fig.7 Effect of the polymerization potential on the elution effect of template molecules (a)0V~0.8V (b)0V~1.0V (c)-0.2V~0.8V (d)-0.2V~1.0V (e)0V~1.2V

Fig.8 The selection of polymerization cycle number

Fig.9 Optimization of template molecule elution time

Fig.10 Optimization of template molecule adsorption time

Fig.11 CV curves of the different electrodes in characterizing solution

Fig.12 SWV curves of the different electrodes in characterization solution

Fig.13 Structural analogues and interfering substances molecular structure (a) Ciprofloxacin (b) Danofloxacin (c) Norfloxacin (d) Tetracycline

Fig.14 Selectivity of molecularly imprinted polymeric membrane electrode (a) Enrofloxacin (b) Ciprofloxacin (c) Danofloxacin (d) Norfloxacin (e) Tetracycline

Fig.15 Relationship curve between peak current difference ΔI and standard solution concentration

Fig.16 SWV curves of the different concentration enrofloxacin standard solution

Table 1 Sample spike recovery and precision (n = 5)


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