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| Research Advances in Recombinase-aided Amplification Technology and Its Application in Rapid Detection of Pathogenic Microorganisms |
MA Qiao-ni1,WANG Meng1,**( ),ZHU Xing-quan2,**() |
1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences,Lanzhou 730046, China
2 College of Veterinary, Shanxi Agricultural University, Taigu 030801, China |
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Abstract The establishment of rapid diagnostic methods for pathogens is of great significance for the prevention and control of animal epidemics and public health safety in China. Recombinase-aided amplification (RAA) is a novel isothermal amplification technology in which rapid amplification of DNA or RNA can be carried out at low temperatures, and the entire RAA reaction is simple, rapid, accurate, power-saving and convenient. There are three core substances of this technology, including recombinase, single-stranded DNA binding protein and DNA polymerase, which can replace the traditional PCR thermal cycle chain decomposition process. Therefore, the RAA technology will play a more and more important role in the detection of pathogenic microorganisms. The research advances in the detection of pathogens by the RAA technology was reviewed, which may provide reference for further relevant studies.
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Received: 15 February 2021
Published: 06 July 2021
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Corresponding Authors:
Meng WANG,Xing-quan ZHU
E-mail: wangmeng02@caas.cn;xingquanzhu1@hotmail.com
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