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| Immobilization and Properties of Lipase from Aspergillus niger on Sol-gels,Hydrophobic Supports |
| LI Gang-rui, LI Lin-li, FAN Xang, MENG Yan-fa |
| College of Life Science, Key Laboratory of Bio-Resources and Eco-Environment Ministry of Education, Sichuan University, Chengdu 610064, China |
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Abstract The study on immobilization of lipase by sol-gel has become research hotspot in recent years.The aim is to find the optimum conditions for the immobilization of lipase from Aspergillus niger and also to select the most suitable support maximizing the immobilized enzyme yield under these optimum conditions.TMOS,MTMS,ETMS,PTMS were used as the precursors in the preparation of encapsulated lipase from Aspergillus niger. The 80.2% of immobilized degree and the 136.3% of relative activity were obtained under optimum conditions, ETMS/TOMS=5:1 and molar ratio of water/silane = 8.With emulsified olive oil as substrate,the kinetics properties for immobilized enzyme were assessed as follows. The Km of immobilized lipase and free lipase in pH 4.0 and 50℃ conditions were detected to be 1.899×10-4 mol/L and 2.789×10-4mol/L by double reciprocal plot,Lineweaver-Burk plot, respectively. The optimum pH of immobilized lipase and free lipase were analyzed to be 4.0 in optimum temperature. The relative activity of immobilized lipase was found above 95% in the range of pH4.0 to pH5.5. The optimum temperature of immobilized lipase was observed at 60℃ which was 10℃ higher than free lipase. The pH stability and thermostability of immobilized lipase were obviously improved in comparison with free enzyme. When immobilized lipase was cyclically used for twelve of reactions, it still retained 71.7% of original activity. When the immobilized lipase was storaged in the dark at room temperature for 180 days, it maintained 79.2% of the initial activity.
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Received: 20 January 2014
Published: 25 April 2014
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