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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2018, Vol. 38 Issue (11): 51-58    DOI: 10.13523/j.cb.20181107
    
Research Advances in Whole-cell Lipases
Hong-qiu SHI,Dai-ming ZHA(),Bing-huo ZHANG,Han-quan LI
School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, China
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Abstract  

Lipases being important industrial enzymes, which have been widely used in many industrial fields. Compared with free lipases and physical or chemical immobilized lipases, whole-cell lipases have the advantages of simple preparation, no protein extraction and purification, natural immobilization, better stability and resistance, low cost of preparation and equipment, etc. Therefore, the utilization of lipases in the form of whole-cell is known as one of the most promising ways to reduce the cost of biotransformation. The study on whole-cell lipases has always been a hot spot in the lipase field. The research advances in whole-cell lipases, including the wild-type whole-cell lipases and the gene engineered whole-cell lipases, were summarized and reviewed. Furthermore, the future research directions of whole-cell lipases were prospected so as to provide a useful reference for the follow-up studies.

Key words： Whole-cell lipase      Gene engineered      wild-type     
Received: 08 August 2018      Published: 06 December 2018
ZTFLH:  Q55  
Corresponding Authors: Dai-ming ZHA     E-mail: dmzha2015@126.com
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Hong-qiu SHI
Dai-ming ZHA
Bing-huo ZHANG
Han-quan LI
Cite this article:

Hong-qiu SHI,Dai-ming ZHA,Bing-huo ZHANG,Han-quan LI. Research Advances in Whole-cell Lipases. China Biotechnology, 2018, 38(11): 51-58.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181107     OR     https://manu60.magtech.com.cn/biotech/Y2018/V38/I11/51

Source Classification Characteristics
野生型全细胞脂肪酶 从自然界中筛选具有全细胞脂肪酶活力的野生型菌株 丝状真菌、细菌和放线菌 制备简单,只需从自然界中筛选即可;来源较丰富;表达效率普遍较低、反应类型较少、反应活性及稳定性较差
基因工程全细胞脂肪酶 通过基因工程手段构建胞内表达或表面展示的基因工程菌 胞内表达型和表面展示型 制备复杂,需通过繁琐的基因工程手段构建;可根据需求选择目的基因和表达宿主;表达宿主来源较少;表达效率较高、反应类型较多、反应活性及稳定性较好
Table 1 Comparisons of wild-type whole-cell lipases and genetic engineered whole-cell lipases
Research directions Research contents
天然全细胞脂肪酶资源的挖掘 极端环境中筛选具有优良酶学性质的天然全细胞脂肪酶
胞内表达全细胞脂肪酶的构建 宿主资源的挖掘、脂肪酶基因资源的挖掘、表达载体的优化构建、细胞透化技术的开发
表面展示全细胞脂肪酶的构建 宿主资源的挖掘、锚定蛋白的开发、连接肽的影响、多蛋白共展示技术的开发、展示载体的优化构建
生物转化领域的应用 反应类型的拓展及其反应条件的优化
Table 2 Future research directions of whole-cell lipases
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