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| Immobilized L-threonine Aldolase by Amino Resin and Its Application |
CHEN Kai-tong1,ZHENG Wen-long1,2,YANG Li-rong1,2,XU Gang1,WU Jian-ping1,2,**( ) |
1 Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
2 Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University,Hangzhou 311200, China |
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Abstract L-threonine aldolase (L-TA) synthesizes β-hydroxy-α-amino from glycine and aldehyde. β-hydroxy-α-amino acids with two chiral centers is an important pharmaceutical intermediate for the synthesis of many drugs. However, free threonine aldolase is difficult to be reused and separated, which seriously hinders industrial application. Immobilization technology can effectively solve these problems. L-threonine aldolase was immobilized by amino carrier NAA and glutaraldehyde was used as the crosslinking reagent. After optimization of conditions, the optimal immobilization conditions were determined as: enzyme loading 13 U, 0.6 g carrier, glutaraldehyde concentration of 0.4 %(V/V), activation time of 2 h, pH of 8.5 and temperature of 35℃, and immobilization time of 5 h. Under these conditions, the activity recovery of immobilized enzyme was 85.7%. The half-life at 30℃ can reach 59 days, which was 6.5 times that of the free enzyme. The immobilized enzyme was applied in the synthesis of L-syn-3-[4-(methylsulfonyl)phenylserine] (L-syn-MTPS) for 460 h still remained activity of 79.4%. Furthermore, the method to reuse carrier was developed. In this method, the residual amino groups on the surface of the inactivated immobilized enzyme were first activated by glutaraldehyde, and then further combined with the fresh enzyme to realize the reuse of the resin. Using this method, the carrier can be reused twice and the prepared immobilized enzyme can still be used for 460 h. This method greatly reduces the cost of immobilization and lays a solid foundation for the industrial application of immobilized L-TA.
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Received: 16 June 2021
Published: 30 September 2021
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Corresponding Authors:
Jian-ping WU
E-mail: wjp@zju.edu.cn
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