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| Recombineering Based on Mycobacteriophage and Its Application |
| FAN Xiang-yu, XIE Jian-ping |
| Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Enviroment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Chongqing 400715, China |
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Abstract Bacteriophage is a powerful tool to address fundamental genetics issues. This is true for Mycobacteriophages too, a well-documented resource for Mycobacterium tuberculosis genetics. Recent developments of mycobacterial recombineering system, which is based on mycobacteriophage Che9c-encoded proteins, are reviewed and its application in basic M. tuberculosis biology is outlined. The advantage of this system is that it is independent of bacterial recA system, restriction endonuclease and DNA ligase, and complex in vitro manipulation. The expression of Che9c-encoded exonuclease and recombinase could substantially complete the construction of gene knockouts or knock-ins, point mutants and mycobacteriophage mutants. The mycobacterial recombineering system is a facile new tool to study gene function and for mutation analysis.
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Received: 06 December 2011
Published: 25 September 2012
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