| Orginal Article |
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| The Research Progress of Gene Cloning and Assembly |
SHENG Xiao-jing,QI Xiao-xue,XU Lei,QI Zhi-qing( ),DIAO Yong() |
| Insititute of Molecular Medicine, School of MEDIcine, Huaqiao University, Quanzhou 362021, China |
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Abstract With the development of sequencing technology, the number of known DNA sequences increase exponentially. In order to explore their biological functions more quickly, some new techniques have been developed. Most of these techniques require to construct plasmids in vitro, but the purification process of recombinant enzymes is complicated, transport and preservation are very difficult, which leads to high cost. Recently, researchers have developed simple and low-cost ways to assemble DNA in vivo. The research status, principle, advantages and disadvantages of all kinds of methods are reviewed and the trend is prospected in combination with practical work, hoping to provide a reference for the further research.
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Received: 11 May 2019
Published: 27 March 2020
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Corresponding Authors:
Zhi-qing QI,Yong DIAO
E-mail: zqqi@hotmail.com;diaoyong@hqu.edu.cn
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