Optimization of separating conditions of mouse gut flora by high performance ion exchange chromatography analysis

China Biotechnology

2007, Vol. 27

Issue (7): 75-79 DOI:

Optimization of separating conditions of mouse gut flora by high performance ion exchange chromatography analysis

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Abstract

The influence of separating effect of different chromatographic conditions of mouse gut flora by high performance ion exchange chromatography analysis was studied. The optimum chromatographic conditions for separating gut bacteria were determined. The sample was applied to the chromatography column packed with Toyopearl SuperQ-650c anion resin, equilibrated with 0.02mol/L piperazin-hydrochloric acid buffer (pH 8.0), and elution salt 1mol/L NaCl, eluted with the gradient of 0-50% NaCl/ 80 min, then 50-75% NaCl/ 25 min at the flow rate 1mL/min, and injecting volume was 1mL.Under these conditions, intestinal flora were separated into several fractions. The establishment of HPLC analysis method will lay a foundation of further research on the components of mouse gut flora and their dynamic changes.

Key words： gut flora high performance ion exchange chromatography separating conditions optimization

Received: 09 February 2007 Published: 25 July 2007

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. Optimization of separating conditions of mouse gut flora by high performance ion exchange chromatography analysis. China Biotechnology, 2007, 27(7): 75-79.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I7/75

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