中国生物工程杂志

［1］ Campbell K H, McWhir J, Wilmut I,et al. Sheep cloned by nuclear transfer from a cultured cell line. Nature , 1996 , 380 : 64~66 ［2］ Cibelli J B, Stice S L, Robl J M,et al. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science ,1998 , 280 : 1256~1258 ［3］ Wakayama T, Zuccotti M, Yanagimachi R, et al. Fulltime development of mice from enucleated oocytes injected with cumulus cell line. Nature , 1998 , 394 : 369~374 ［4］ Baguisi A, Behboodi E , Echelard Y, et al. Production of goats by somatic cell nuclear transfer. Nat Biotechnol, 1999 , 17:456~461 ［5］ Polejaeva I A, Chen S H, Vaught T D, et al. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature, 2000 407 : 86~90 ［6］ Shin T, Kraemer D, Pryor J,et al. A cat cloned by nuclear transplantation. Nature , 2002 , 415(6874):859 ［7］ Chesné P, Adenot P G, Viglietta C, et al. Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat Biotechnol. 2002,20(4):366~369 ［8］ Woods G L , White K L, Li G P, et al. A mule cloned from fetal cells by nuclear transfer. Science , 2003 , 301 : 1063 ［9］ Zhou Q, Renard J P, Le Friec G, et al. Generation of fertile cloned rats by regulating coyote activation. Science , 2003 , 302 : 1179 ［10］ Hwang W S,Lee B C, Kim M K, et al.Dogs cloned from adult somatic cells. Nature 2005 ,436(7051):641 ［11］ Tatham B G, Dowsing A T, Trounson A O. Enucleation by centrifugation of in vitromatured bovine oocytes for use in nuclear transfer. Biol Reprod,1995, 53:1088~1094 ［12］ Fulka Jr J, Moor R M. Noninvasive chemical enucleation of mouse oocytes. Mol Reprod Dev, 1993,34: 427~430 ［13］ Galli C, Lagutina I, Lazzari G.Introduction to cloning by nuclear transplantation. Clon Stem Cells, 2003,5:223~232 ［14］ Kim T M, Hwang W S, Shin J H, et al. Development of a nonmechanical enucleation method using Xray irradiation in somatic cell nuclear transfer. Fertil Steril, 2004, 82: 963~965 ［15］ Wagoner E J, Rosenkrans C F, Gliedt D W, et al.Functional enucleation of bovine oocytes:effects of centrifugation and ultraviolet light. Theriogenology, 1996 ,46:279~284 ［16］ Kawakami M, Tani T, Yabuuchi A, et al . Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes. Clon Stem Cells, 2003,5: 379~387 ［17］ Tani T, Shimada H, Kato Y,et al . Demecolcineassisted enucleation for bovine cloning .Cloning and Stem Cells, 2006, 8(1): 61~66 ［18］ Elsheikh A S, Takahashi Y, Katagiri S, et al. Functional enucleation of mouse metaphase II oocytes with etoposide. Jpn J Vet Res, 1998 , 45 (4):217~220 ［19］ Baguisi A, Overstrom E W. Induced enucleation in nuclear transfer procedures to produce cloned animals.Theriogenology, 2000,54: 209 ［20］ Gasparrini B. Attempted chemical enucleation of in vitro matured sheep oocytes after activation and their use in nuclear transfer.Theriogenology, 2002,57:414 ［21］ Yin X J, Tani T, Yonemura I, et al. Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes. Biol Reprod,2002 , 67(2):442~446 ［22］ Russell D F, Ibánez E, Albertini D F, et al. Activated bovine cytoplasts produced by induced enucleation support development of bovine nuclear transfer embryos in vitro. Mol Reprod Dev, 2005,72(2):161~170 ［23］ Gasparrini B, Gao S, Ainslie A, et al. Cloned mice derived from embryonic stem cell .karyoplasts and activated cytoplasts prepared by induced enucleation. Biol Reprod, 2003 , 68 (4): 1259~1266 ［24］ Xi J Y, Kato Y, Tsunoda Y.Effect of delayed enucleation on the developmental potential of nuclear transfered oocytes receiving adult and fetal fibroblast cells. Zygote, 2002 , 10 (3) : 217~222 ［25］ Miyoshi K, Mori H, Yamamoto H, et al. Effect of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro .Journal of Reproduction and Development, 2008,54（2）:117~121 ［26］ Ibáez E, Albertini D F, Overstrm EW. Demecolcineinduced oocyte enucleation for somatic cell cloning: oordination between cellcycle egress, kinetics of cortical cytoskeletal interactions, and second polar body extrusion. Biol Reprod, 2003, 68: 1249~1258 ［27］ Liu J L, Sung L Y, Barber M, et al. Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes. Biol Reprod, 2002, 66: 1342~1349 ［28］ Moura M T, de Sousa R V, de Oliveira Leme L, et al .Analysis of actinomycin D treated cattle oocytes and their use for somatic cell nuclear transfer .Animal Reproduction Science 2007,9（online）