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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2015, Vol. 35 Issue (9): 122-127    DOI: 10.13523/j.cb.20150917
综述     
解淀粉芽孢杆菌高效合成胞苷的代谢调控机制及育种策略
吴庆, 刘慧燕, 方海田, 何建国, 贺晓光, 于丽男, 王梦娇
宁夏大学农学院 银川 750021
Metabolic Control Fermentation Mechanism and Breeding Strategies of Cytidine Excessive Biosynthesis in Bacillus amyloliquefaciens
WU Qing, LIU Hui-yan, FANG Hai-tian, HE Jian-guo, HE Xiao-guang, YU Li-nan, WANG Meng-jiao
School of Agriculture Ningxia University, Yinchuan 750021, China
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摘要：

胞苷是合成抗病毒、抗肿瘤药物的良好中间体,也是核苷酸类保健食品和功能性食品的重要原料.主要论述了解淀粉芽孢杆菌高效合成胞苷的代谢调控机制和构建胞苷高产菌株的育种策略.重点阐述了通过提高解淀粉芽孢杆菌嘧啶操纵子转录水平,增强胞苷合成代谢途径、阻断胞苷降解途径、增大磷酸戊糖途径向胞苷合成途径的分流量、提高胞苷合成前端代谢物PRPP的合成量、增强中心碳代谢流向胞苷合成途径、减少旁路代谢途径及促进胞苷的分泌等方法,选育出胞苷高产菌株,不仅为胞苷高产菌株的选育提供参考,也为解决目前胞苷工业化生产中存在的问题提供思路.
关键词： 解淀粉芽孢杆菌;  胞苷;  代谢调控机制;  育种策略    
Abstract:

Cytidine is good intermediates of anti-tumor and antiviral, also can be used as health food ingredients.The metabolic regulatory mechanism of cytidine biosynthesis in Bacillus amyloliquefaciens was discussed and the breeding strategy of constructing cytidine high-yielding strain was summarized. The focuses were on elaborating the six breeding strategies, such as improving the pyrimidine operon transcriptional level of Bacillus amyloliquefaciens, enhancing cytidine anabolic pathways and central metabolic pathways to cytidine synthesis, blocking degradation pathway of cytidine, increasing the pentose phosphate pathway to the sub-flow of cytidine synthesis pathway, reducing the bypass pathway and accelerating cytidine secretion.Thereby, the strategies of breeding high yield strain were proposed, which could provide some ideas for breeding cytidine high-yielding strains, and solve the existing problems in the processing of cytidine industrial production.
Key words: Bacillus amyloliquefaciens    Cytidine    Metabolic regulation mechanism    Breeding strategy
收稿日期: 2015-03-25 出版日期: 2015-09-25
ZTFLH:  Q819  
基金资助:

国家自然科学基金资助项目(31301542)
通讯作者: 方海田     E-mail: fanght@nxu.edu.cn
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引用本文:

吴庆, 刘慧燕, 方海田, 何建国, 贺晓光, 于丽男, 王梦娇. 解淀粉芽孢杆菌高效合成胞苷的代谢调控机制及育种策略[J]. 中国生物工程杂志, 2015, 35(9): 122-127.

WU Qing, LIU Hui-yan, FANG Hai-tian, HE Jian-guo, HE Xiao-guang, YU Li-nan, WANG Meng-jiao. Metabolic Control Fermentation Mechanism and Breeding Strategies of Cytidine Excessive Biosynthesis in Bacillus amyloliquefaciens. China Biotechnology, 2015, 35(9): 122-127.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150917    或    https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I9/122


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