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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2015, Vol. 35 Issue (9): 71-77    DOI: 10.13523/j.cb.20150911
综述     
Alexa Fluor荧光标记在细胞学和分子生物学研究中的应用
王洪苏, 关桂静, 刘金香
西南大学柑桔研究所 中国农业科学院柑桔研究所 重庆 400712
Application of Alexa Fluor in Cytology and Molecular Biology
WANG Hong-su, GUAN Gui-jing, LIU Jin-xiang
Citrus Research Institute, SouthWest University, Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China
 全文: PDF(426 KB)   HTML
摘要:

Alexa Fluor染料为罗丹明或香豆素的衍生物,是一种含有活性基团的有机荧光染料,具有激发光谱窄、发射光谱宽、量子产率高、光稳定性好,以及受温度、pH影响小等优点.近年来,Alexa Fluor染料作为荧光探针在细胞学和分子生物学研究领域取得了广泛应用,但对Alexa Fluor染料进行系统阐述的文章很少.因此,在综述Alexa Fluor染料的基本特征和优缺点的基础上,阐述其在细胞学和分子生物学研究中的应用,并对其应用和发展方向进行展望.

关键词: 荧光标记Alexa Fluor细胞学分子生物学    
Abstract:

Alexa Fluor is a derivative of rhodamine or coumarin. As an organic fluorescent dye, Alexa Fluor has some obvious advantages such as narrow excitation spectrum, wide emission spectrum, high quantum yield, good light stability and less temperature and pH impact. Recently, though Alexa Fluor dye gets a wide range of applications in the field of cytology and molecular biology research, there are few articles about Fluor Alexa dyes. elaborate Alexa Fluor's application and make prospects for its development in cytology and molecular biology science based on the characteristics are elaborated.

Key words: Fluorescently labeled    Alexa Fluor    Cytology    Molecular biology
收稿日期: 2015-04-29 出版日期: 2015-09-25
ZTFLH:  Q819  
基金资助:

重庆自然科学基金资助项目(CSTC2012JJA80036)

通讯作者: 刘金香     E-mail: ljinxiang@126.com
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王洪苏, 关桂静, 刘金香. Alexa Fluor荧光标记在细胞学和分子生物学研究中的应用[J]. 中国生物工程杂志, 2015, 35(9): 71-77.

WANG Hong-su, GUAN Gui-jing, LIU Jin-xiang. Application of Alexa Fluor in Cytology and Molecular Biology. China Biotechnology, 2015, 35(9): 71-77.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150911        https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I9/71


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