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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2022, Vol. 42 Issue (12): 91-100    DOI: 10.13523/j.cb.2204068
综述     
解脂耶氏酵母基于木质纤维素原料生产化学品研究进展*
王露鑫1,2,房立霞1,陈雅如1,李孟旭1,牛小龙1,2,宋浩1,2,曹英秀1,**()
1 天津大学化工学院 教育部合成生物学前沿科学中心 系统生物工程教育部重点实验室 天津 300072
2 天津大学青岛海洋工程技术研究院 青岛 266237
Advances in the Production of Chemicals from Lignocellulosic Material by Yarrowia lipolytica
WANG Lu-xin1,2,FANG Li-xia1,CHEN Ya-ru1,LI Meng-xu1,NIU Xiao-long1,2,SONG Hao1,2,CAO Ying-xiu1,**()
1 Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2 Qingdao Institute for Ocean Engineering of Tianjin University, Qingdao 266237, China
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摘要：

解脂耶氏酵母具有遗传背景清晰、分子操作体系较为成熟、抗逆性强、底物谱广、有机酸和蛋白质分泌能力强等优点,在微生物发酵生产化学品领域极具应用潜力。木质纤维素是丰富的可再生生物质资源,以木质纤维素原料替代化石原料生产化学品对于缓解全球能源危机、保障粮食安全等意义重大。解脂耶氏酵母可以天然代谢木质纤维素水解产生的葡萄糖,但对其他水解产物(如木糖)的利用效率极低。综述解脂耶氏酵母利用木质纤维素原料的代谢途径及改造策略,以木质纤维素原料生产化学品为例,重点讨论该过程中的主要瓶颈问题及解决办法,为后续研究提供参考。
关键词： 解脂耶氏酵母;  木质纤维素;  木糖;  代谢工程    
Abstract:

Yarrowia lipolytica has great application potential in the field of microbial fermentation of chemicals due to its clear genetic background, relatively mature molecular manipulation system, strong stress resistance, broad substrate spectrum, and strong organic acid and protein secretion capabilities. Lignocellulose is the most abundant renewable biomass resource on the earth, and the use of lignocellulose materials to replace fossil materials to produce chemicals is of great significance in alleviating global energy crisis and ensuring food security. Y. lipolytica can naturally metabolize glucose produced by hydrolysis of lignocellulose. However, the utilization efficiency of other hydrolysis products such as xylose is extremely low. This article reviews the metabolic pathways and engineering strategies of Y. lipolytica using lignocellulosic materials, as well as examples of using lignocellulosic materials to produce chemicals, and focuses on the bottlenecks in this process. The solutions to these bottlenecks were also discussed, with the aim to provide useful information for relevant studies in this field.
Key words: Yarrowia lipolytica    Lignocellulose    Xylose    Metabolic engineering
收稿日期: 2022-04-26 出版日期: 2023-01-05
ZTFLH:  Q939  
基金资助: *国家重点研发计划(2021YFC2104400);国家自然科学基金(22078240);天津市自然科学基金(19JCQNJC09200);天津市青年人才托举工程资助项目(TJSQNTJ-2018-16)
通讯作者: 曹英秀     E-mail: caoyingxiu@tju.edu.cn
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王露鑫
房立霞
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李孟旭
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宋浩
曹英秀

引用本文:

王露鑫,房立霞,陈雅如,李孟旭,牛小龙,宋浩,曹英秀. 解脂耶氏酵母基于木质纤维素原料生产化学品研究进展*[J]. 中国生物工程杂志, 2022, 42(12): 91-100.

WANG Lu-xin,FANG Li-xia,CHEN Ya-ru,LI Meng-xu,NIU Xiao-long,SONG Hao,CAO Ying-xiu. Advances in the Production of Chemicals from Lignocellulosic Material by Yarrowia lipolytica. China Biotechnology, 2022, 42(12): 91-100.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2204068    或    https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I12/91

图1  木质纤维素中糖的种类
图2  工程化解脂耶氏酵母降解纤维素过程
图3  解脂耶氏酵母利用纤维素、葡萄糖、木糖、半乳糖、阿拉伯糖、乙酸盐的代谢途径以及有机酸、脂质、萜烯类化学品合成路径
化学品 原料 菌株 遗传修饰 产量 参考文献
柠檬酸 纤维素 SWJ-1b - 42.4 g/L [32]
柠檬酸 半乳糖 Y4588 gal1、gal7、gal10m、gal10e 29.2 g/L [49]
柠檬酸 半乳糖 Y4779 Δmfe2、gpd1、dga1、hxk1、yht3、suc2、inu1 23 g/L [65]
柠檬酸 纤维二糖 Po1f-BC cdt-1、gh1-1 5.1 g/L [28]
柠檬酸 木糖 XYL+Obese Δpox1-6、Δtgl4、gpd1、dga2、xyl1、xyl2、xyl3 80 g/L [37]
α-酮戊二酸 纤维素 Y. lipolytica - 8.33 g/L [29]
琥珀酸 木糖 PSA02004PP xyl1、xyl2、xyl3 11.2 g/L [38]
琥珀酸 木糖/葡萄糖 PSA02004 - 28.2 g/L [40]
脂质 纤维素 CYLpO bgl1、bgl2、eg1、eg2、cbh1、cbh2、scd1、dga1 19 g/L [31]
脂质 纤维素 YL163t acl dga1 snf1::cbh1-cbh2-eg2 32 mg/g avicel [66]
脂质 半乳糖 Y4588 gal1、gal7、gal10m、gal10e 3.22 g/L [49]
脂质 半乳糖 YLZ150 Δtgl4、Δpox1-6、gpd1、dga2、hxk1、gal1、gal7、gal10m、gal10e、suc2、inu1 23.82 g/L [65]
脂质 木糖 E26 XUS xyl1、xyl2 15.06 g/L [42]
脂质 纤维素水解液 ylXYL+Obese-XA Δpox1-6、Δtgl4、gpd1、dga2、xyl1、xyl2、xyl3、xpka、ack 16.5 g/L [67]
脂质 木糖 YSXID Δpex10::dga1 xylA、xk 12.01 g/L [68]
脂质 木糖 XYL+Obese Δpox1-6、Δtgl4、gpd1、dga2、xyl1、xyl2、xyl3 20.1 g/L [37]
脂质 木糖/甘油 XYL+Obese Δpox1-6、Δtgl4、gpd1、dga2、xyl1、xyl2、xyl3 50.5 g/L [37]
脂质 Acetate/甘油 PO1f-acsSP-acc1-fas2-1 acsSP、acc1SP、fas2-2SP 41.72% [53]
脂质 乙酸盐/乙酸 MTYL065 acc1、dga1 115 g/L [54]
柠檬烯 木糖/葡萄糖 YBX08 xyl1、xyl2、xyl3、tls、tndps、hmg1、erg12 19.4 mg/L [43]
原人参二醇 木糖 Y14 xyl1、xyl2、xyl3、ds、ppds-linker-atr1、tHMG1、erg9、erg20、tkl、tal、tx 300.63 mg/L [44]
表1  解脂耶氏酵母利用木质纤维素原料生产化学品
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