微生物合成根皮素及其糖苷研究进展 <sup>*</sup>

doi:10.13523/j.cb.2005070

中国生物工程杂志

2020, Vol. 40

Issue (10): 76-84 DOI: 10.13523/j.cb.2005070

综述

微生物合成根皮素及其糖苷研究进展 ^*

刘金丛¹,刘雪¹,於洪建²,赵广荣^1,^**(

)

1 天津大学化工学院教育部合成生物学前沿科学中心系统生物工程教育部重点实验室天津 300072
2 天津市益倍生物科技集团天津 300450

Recent Advances in Microbial Production of Phloretin and Its Glycosides

LIU Jin-cong¹,LIU Xue¹,YU Hong-jian²,ZHAO Guang-rong^1,^**(

)

1 School of Chemical Engineering and Technology, Tianjin University, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin 300072, China
2 Ubasio Biotech Go.,Ltd., Tianjin 300450, China

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摘要：

根皮素及其糖苷是二氢查耳酮类天然产物,具有抗氧化、抗炎和抑菌等多种生理活性,可应用于食品、药品、化妆品等行业。目前,根皮素及其糖苷主要从植物中提取,但含量低、组分复杂制约了高效、低成本的分离制备。随着合成生物学的发展,工程微生物生产根皮素及其糖苷将是一种有潜力的方法。综述了微生物合成根皮素及其糖苷的关键基因鉴定、代谢途径重构和优化策略,并针对酶的特异性差、副产物多等问题提出了技术对策。

关键词： 根皮素; 根皮素糖苷; 微生物合成; 代谢工程; 合成生物学

Abstract:

Phloretin and its glycosides are natural dihydrochalcones with various physiological activities, such as antioxidant, anti-inflammatory and anti-bacterial activities, and have potential application in food, pharmaceutical, and cosmetics industries. At present, phloretin and its glycosides are mainly extracted from plants. However, the low content and complex components make it difficult to prepare products with high efficiency and low cost. With the development of metabolic engineering and synthetic biology, microbial production of phloretin and its glycosides has become an alternative approach. This article reviews the identification of key genes, the reconstruction of synthetic pathways and optimization strategies for the biosynthesis of phloretin and its glycosides. Finally, the potential strategies to solve existing problems as unspecific enzymes and the formation of multiple byproducts were proposed.

Key words: Phloretin Phloretin-glycosides Microbial production Metabolic engineering Synthetic biology

收稿日期: 2020-05-31 出版日期: 2020-11-10

ZTFLH:

Q819

基金资助: * 国家自然科学基金(31870077)

通讯作者: 赵广荣 E-mail: grzhao@tju.edu.cn

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引用本文:

刘金丛,刘雪,於洪建,赵广荣. 微生物合成根皮素及其糖苷研究进展 ^*[J]. 中国生物工程杂志, 2020, 40(10): 76-84.

LIU Jin-cong,LIU Xue,YU Hong-jian,ZHAO Guang-rong. Recent Advances in Microbial Production of Phloretin and Its Glycosides. China Biotechnology, 2020, 40(10): 76-84.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2005070 或 https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I10/76

图1 根皮素及其糖苷化合物结构

图2 根皮素和柚皮素生物合成途径

图3 代谢工程微生物合成根皮素

表1 微生物合成根皮素

图4 根皮素糖基转移酶的系统发育树

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