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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2022, Vol. 42 Issue (4): 93-101    DOI: 10.13523/j.cb.2110013
综述     
革兰氏阴性菌TonB依赖性受体的功能与结构研究进展*
王娅玲1,2,3,程安春1,2,3,刘马峰1,2,3,**()
1 四川农业大学动物医学院预防兽医研究所 成都 611130
2 四川农业大学动物医学院禽病防治中心 成都 611130
3 动物疫病与人类健康四川省重点实验室 成都 611130
A Review on Structure and Functions of TonB-dependent Receptors in Gram-negative Bacteria
WANG Ya-ling1,2,3,CHENG An-chun1,2,3,LIU Ma-feng1,2,3,**()
1 Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China
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摘要：

为维持生长所需,革兰氏阴性菌需要从外界摄取多种营养物质。分子量小于600 Da的分子可以通过自由扩散的方式通过革兰氏阴性菌的外膜,而大分子物质则需要特殊的转运系统才能将其转运至革兰氏阴性菌的胞内。革兰氏阴性菌对大分子营养物质的识别和转运主要由TonB依赖性受体负责完成。所有革兰氏阴性菌中均有TonB依赖性受体的存在,然而不同种类的革兰氏阴性菌拥有TonB依赖性受体的数量不同且功能各异。最近研究表明,TonB依赖性受体不仅参与了铁、血红素、锰、锌、镍、维生素、碳水化合物等多种营养物质的摄取,而且参与了蛋白酶的分泌。为对TonB依赖性受体提供更为深入和系统的理解,详细介绍了目前已知的TonB依赖性受体的功能及结构,以期为更进一步探知TonB依赖性受体未知功能提供可参考依据。
关键词： 革兰氏阴性菌;  TonB依赖性受体;  功能    
Abstract:

Bacteria need to absorb a variety of nutrients from the outer environments for their survival and replication. The small molecules less than 600 Da can freely pass the outer membrane of the gram-negative bacteria. However, for the transportation of the large size of nutrients, the TonB-dependent receptors of gram-negative bacteria are required. TonB-dependent receptors have been found to be present in all the sequenced gram-negative bacteria, but the number of TonB dependent receptors and their functions are various in different bacteria. It has been shown that TonB-dependent receptors were not only involved in the transportation of many different nutrients, such as iron, heme, manganese, zinc, vitamin, and carbohydrate, but also the secretion of protease. This review provided detailed information about the functions and structure of TonB-dependent receptors from different gram-negative bacteria. It will be helpful for the further understanding of the new functions of TonB-dependent receptors.
Key words: Gram-negative bacteria    TonB-dependent receptor    Function
收稿日期: 2021-10-13 出版日期: 2022-05-05
ZTFLH:  Q819  
基金资助: * 国家自然科学基金(32172851);四川省科技计划应用基础研究项目(2020YJ0344)
通讯作者: 刘马峰     E-mail: liumafengra@163.com
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王娅玲,程安春,刘马峰. 革兰氏阴性菌TonB依赖性受体的功能与结构研究进展*[J]. 中国生物工程杂志, 2022, 42(4): 93-101.

WANG Ya-ling,CHENG An-chun,LIU Ma-feng. A Review on Structure and Functions of TonB-dependent Receptors in Gram-negative Bacteria. China Biotechnology, 2022, 42(4): 93-101.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2110013    或    https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I4/93

Organism(s) Name Substrate Function PDB ID Reference
Escherichia coli FhuA Iron Transport Fe3+ 1BY3 [53]
FepA Iron Transport Fe3+ 1FEP [54]
FecA Iron Transport Fe3+ 1KMO [55]
BtuB Vitamin B12 Transport Vitamin B12 1NQE [56]
Cir — — 2HDF [57]
Fiu Iron Transport Fe3+ 6BPM [58]
YddB Ferredoxin Transport Ferredoxin 6OFR [59]
YncD — — 6V81 [60]
Pseudomonas aeruginosa FptA Iron Transport Fe3+ 1XKW [61]
FpvA Iron Transport Fe3+ 2W75 [62]
PirA Siderophore-drug Transport siderophore-drug molecules 5FP2 [63]
PiuA Siderophore-drug Transport siderophore-drug molecules 5FOK [63]
PiuD Siderophore-drug Transport siderophore-drug molecules 5NEC [64]
PfeA Iron Transport Fe3+ 5M9B [65]
OprC Copper Transport Copper 6FOK —
FoxA Iron Transport Fe3+ 6I98 [66]
Yersinia pestis FyuA Iron Transport Fe3+ 4EPA [67]
Neisseria TbpA Transferrin Transport Fe3+ 3V89 [68]
FrpB Iron Transport Fe3+ 4B7O [69]
ZnuD Zinc Transport zinc 4RVW [70]
Pectobacterium carotovorum FusA Ferredoxin Transport Ferredoxin 4ZGV [71]
Serratia marcescens HasR Heme Transport heme 3CSN [26]
Porphyromonas gingivalis RagA oligopeptide Transport oligopeptide 6SLI [72]
Bacteroides polymorpha SusC Starch Transport oligosaccharide 5T3R [73]
Shigella dysenteriae ShuA Heme Transport heme 3FHH [74]
表1  已知革兰氏阴性菌TonB依赖性受体晶体结构汇总
图1  TonB依赖性受体FepA结构(PDB ID:1FEP)
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