毛蕊花糖苷的生物合成研究进展

doi:10.13523/j.cb.2102012

中国生物工程杂志

2021, Vol. 41

Issue (5): 94-104 DOI: 10.13523/j.cb.2102012

综述

毛蕊花糖苷的生物合成研究进展

翟君叶¹,成旭¹,孙泽敏²,李春³,吕波^1,^*(

)

1 北京理工大学化学与化工学院北京 102488
2 北京理工大学生命学院北京 100081
3 清华大学化学工程系北京 100084

Current Advances in Biosynthesis of Acteoside

ZHAI Jun-ye¹,CHENG Xu¹,SUN Ze-min²,LI Chun³,LV Bo^1,^*(

)

1 School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, China
2 School of Life Sciences, Beijing Institute of Technology, Beijing 100081, China
3 Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

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摘要：

毛蕊花糖苷是由咖啡酸、羟基酪醇、葡萄糖和鼠李糖组成的苯乙醇苷类衍生物,广泛存在于多种天然药用植物中,具有抗炎、抗菌、抗病毒、抗肿瘤、抗氧化、镇痛、神经保护、改善性功能、免疫调节和改善细胞记忆等多方面的生物活性。然而,植物提取来源的毛蕊花糖苷存在含量低、环境污染问题,无法实现绿色可持续的规模化生产。利用合成生物学成功构建了一系列植物天然产物的微生物细胞工厂,为高效生产毛蕊花糖苷提供了新思路。综述了毛蕊花糖苷细胞工厂的研究现状,包括苯乙醇苷生物合成关键代谢途径解析、关键基因元件挖掘和优化、关键前体物的合成等方面,以期为毛蕊花糖苷的生物合成奠定基础。

关键词： 苯乙醇苷; 毛蕊花糖苷; 途径解析; 生物合成

Abstract:

Acteoside is a natural product widely found in many plants, which is composed of caffeic acid, hydroxytyrosol, glucose and rhamnose, including anti-inflammatory, antibacterial, antiviral, anti-tumor, antioxidant, analgesic, neuroprotective, improving sexual function, immunomodulatory, and memory protective activities. However, traditional extraction of acteoside and its derivatives from plants have some problems such as low content, low extraction efficiency and environmental pollution.In recent years, the study of heterologous synthesis of plant natural products has been successfully constructed by the rapid development of synthetic biology, which provides a new idea for the efficient production of acteoside. The recent advances of acteoside cell factory which includes the key metabolic biosynthesis elucidations of phenylethanoid glycoside , mining and optimization of key gene components, synthesis of key precursors were summarized.

Key words: Phenylethanoid glycoside Acteoside Pathway analysis Biosynthesis

收稿日期: 2021-02-08 出版日期: 2021-06-01

ZTFLH:

Q815

通讯作者: 吕波 E-mail: lv-b@bit.edu.cn

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引用本文:

翟君叶,成旭,孙泽敏,李春,吕波. 毛蕊花糖苷的生物合成研究进展[J]. 中国生物工程杂志, 2021, 41(5): 94-104.

ZHAI Jun-ye,CHENG Xu,SUN Ze-min,LI Chun,LV Bo. Current Advances in Biosynthesis of Acteoside. China Biotechnology, 2021, 41(5): 94-104.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2102012 或 https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I5/94

图1 毛蕊花糖苷的结构和含量分布

图2 毛蕊花糖苷的生物合成途径解析

表1 苯乙醇苷转录组数据分析结果

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